沙格列汀对ox-LDL诱导的血管内皮细胞损伤及miR-590/TLR4/NF-κB表达的影响

Effects of saggliptin on vascular endothelial cell injury induced by ox-LDL and expression of miR-590/TLR4/NF-κB

目的探讨沙格列汀对ox-LDL诱导的血管内皮细胞损伤及miR-590/TLR4/NF-κB表达的影响。方法培养人脐静脉内皮细胞(HUVEC)并分为对照组、ox-LDL组、沙格列汀组、沙格列汀+miR-590抑制物组、NC模拟物组、miR-590模拟物组、NC抑制物组、miR-590抑制物组。检测细胞的增殖活力、细胞中miR-590/TLR4/NF-κB表达量及培养基中肿瘤坏死因子α(TNF-α)、白细胞介素1β(IL-1β)、细胞间黏附分子1(ICAM-1)、血管细胞黏附分子1(VCAM-1)的含量。结果ox-LDL组细胞中TLR4、NF-κB p65表达量及培养基中TNF-α、IL-1β、ICAM-1、VCAM-1的含量均明显高于对照组,细胞增殖活性及细胞中miR-590的表达量明显低于对照组;沙格列汀组细胞中TLR4、NF-κB p65表达量及培养基中TNF-α、IL-1β、ICAM-1、VCAM-1的含量均明显低于ox-LDL组,细胞增殖活性及细胞中miR-590的表达量明显高于ox-LDL组;沙格列汀+miR-590抑制物组细胞中TLR4、NF-κB p65表达量及培养基中TNF-α、IL-1β、ICAM-1、VCAM-1的含量均明显高于沙格列汀组,细胞增殖活性及细胞中miR-590的表达量明显低于沙格列汀组;miR-590模拟物组细胞中TLR4、NF-κB p65的表达量及培养基中TNF-α、IL-1β、ICAM-1、VCAM-1的含量均明显低于NC模拟物组,miR-590抑制物组细胞中TLR4、NF-κB p65的表达量及培养基中TNF-α、IL-1β、ICAM-1、VCAM-1的含量均明显高于NC抑制物组。结论沙格列汀能够通过miR-590/TLR4/NF-κB通路减轻ox-LDL诱导的血管内皮细胞损伤。

Aim To investigate the effects of saggliptin on vascular endothelial cell injury induced by ox-LDL and expression of miR-590/TLR4/NF-κB.Methods Human umbilical vein endothelial cells(HUVEC)were cultured and divided into control group,ox-LDL group,sagliptin group,sagliptin+miR-590 inhibitor group,NC mimic group,miR-590 mimic group,NC inhibitor group and miR-590 inhibitor group.The proliferation activity,the expression of miR-590/TLR4/NF-κB and the contents of tumor necrosis factor-α(TNF-α),interleukin-1β(IL-1β),intercellular adhesion molecule-1(ICAM-1)and vascular cell adhesion molecule-1(VCAM-1)in culture medium were measured.Results The expression of TLR4,NF-κB p65 in cells and the contents of TNF-α,IL-1β,ICAM-1,VCAM-1 in culture media of ox-LDL group were significantly higher than those of control group,cell proliferation activity and the expression of miR-590 in cells was significantly lower than that of control group;the expression of TLR4,NF-κB p65 in cells and the contents of TNF-α,IL-1β,ICAM-1,VCAM-1 in culture media of sagliptin group were significantly lower than those of ox-LDL group,cell proliferation activity and the expression of miR-590 was significantly higher than that of ox-LDL group;the expression of TLR4,NF-κB p65 in cells and the contents of TNF-α,IL-1β,ICAM-1,VCAM-1 in culture media of saglitine+miR-590 inhibitor group were significantly higher than those in saglitine group,cell proliferation activity and the expression of miR-590 was significantly lower than that of saglitine group;the expression of TLR4,NF-κB p65 in cells and the contents of TNF-α,IL-1β,ICAM-1,VCAM-1 in culture media of miR-590 mimic group were significantly higher than those of NC mimic group,the expression of TLR4,NF-κB p65 in cells and the contents of TNF-α,IL-1β,ICAM-1,VCAM-1 in culture media of miR-590 inhibitor group were significantly lower than those of NC inhibitor group.Conclusion Sagliptin can alleviate ox-LDL-induced vascular endothelial cell injury by the miR-590/TLR4/NF-κB pathway.