香参壳方对人胃癌细胞上皮间质转化及转移的影响

Protective Effect of Xiangshenke Formula on the Epithelial Mesenchymal Transformation and Metastasis of Human Gastric Carcinoma Cells

目的考察香参壳方调节人胃癌细胞SGC-7901上皮间质转化(EMT)及转移的作用机制。方法体外培养SGC-7901细胞,随后加入不同浓度的香参壳方含药血清进行干预。划痕实验及Transwell小室法观察SGC-7901细胞的迁移与侵袭能力,MTT法考察SGC-7901细胞的增殖能力,Western blot法检测细胞中N-cadherin、Vimentin、E-cadherin、p-GSK3β/GSK3β、β-Catenin表达水平。结果香参壳方可有效抑制SGC-7901细胞的迁移及侵袭能力,抑制其增殖,同时下调细胞中N-cadherin、Vimentin、p-GSK3β/GSK3β及β-Catenin的表达,上调E-cadherin的表达。结论香参壳方主要通过介导GSK3β/β-Catenin信号通路抑制胃癌细胞的EMT,降低弱细胞迁移与侵袭能力,从而发挥其胃癌术后的辅助治疗作用。

OBJECTIVE To investigate the mechanism of Xiangshenke formula in regulating the proliferation, migration and EMT of human gastric carcinoma cells SGC-7901. METHODS SGC-7901 cells were cultured in vitro, and then different concentrations of Xiangshenke formula containing serum were added into the cells. Wound healing test and transwell chamber assay were used to observe the SGC-7901 cell migration and invasion. MTT assay was used to investigate the SGC-7901 cell proliferation. Western blot was used to detect the expression levels of N-cadherin, Vimentin, E-cadherin, p-GSK3β/GSK3β and β-Catenin. RESULTS Xiangshenke formula effectively inhibited the migration and invasion of SGC-7901 cells, suppressed the cell proliferation, down-regulated the expression of N-cadherin, Vimentin, p-GSK3β/GSK3β and β-Catenin, and up-regulated the expression of E-cadherin. CONCLUSION Xiangshenke formula can inhibit the SGC-7901 cell epithelial-mesenchymal transition by regulating the GSK3β/β-Catenin signaling pathway, and suppress their migrtaion and invasion ability. It plays an important role in adjuvant therapy after gastric cancer surgery.