磁场作用下葡萄糖近红外光谱检测技术研究

Determination of Glucose by NIR Spectroscopy Under Magnetic Field

采用傅立叶变换红外光谱仪(FTIR)研究了磁场强度对葡萄糖溶液近红外光谱的影响,发现磁场作用下葡萄糖的近红外光谱吸收强度和部分峰位发生显著变化。分析了磁场对葡萄糖溶液近红外光谱吸收的影响机理。采用偏最小二乘回归法(PLS)建立了磁场作用下葡萄糖溶液的定量分析模型,使用验证集进行验证。研究结果表明,磁场对葡萄糖分子基团偶极矩产生诱导作用,使偶极矩增大,吸收增强;同时磁场作用下,葡萄糖分子趋于沿平行于磁场的方向排列,其基团振动频率(特征吸收峰)吸光度与浓度变化的线性关系得到极大的改善。该研究有助于提高葡萄糖分子吸收强度及其测量精度,为进一步提高血糖检测精度提供技术支持。

Near-infrared(NIR)spectroscopy for measuring blood glucose concentration has great significance in biomedicine.In this paper,Fourier transform infrared(FTIR)spectroscopy was used to study the influence of different magnetic field strengths on the near-infrared spectra of different concentrations of glucose solution.The near-infrared absorption intensity and partial peak position of glucose under magnetic field were observed to change significantly.The influence mechanism of magnetic field on the absorption of near-infrared spectrum of glucose solution was systematically analyzed.A quantitative analysis model for glucose solution under magnetic field was established by partial least squares regression(PLS).The calibration set was used to verify the calibration coefficient of glucose solution.Experimental results showed that the magnetic field induces the dipole moment of the glucose molecular group,leading to the dipole moment of the molecule and its absorption increasing,while the glucose molecules tend to align along the direction parallel to the magnetic field under the action of magnetic field,and the consistency of the linear relationship between absorbance and concentration change of molecular vibration frequency is greatly improved.This study helps to improve the absorption intensity of glucose molecules and its measurement accuracy,and provides a technical support for further improving the accuracy for blood glucose detection.