熊胆粉对运动性疲劳大鼠的抗疲劳作用研究

Study on the Anti-fatigue Effect of Bear Bile Powder in Rats with Exercise-induced Fatigue

目的:分析熊胆粉用于运动性疲劳大鼠抗疲劳作用及其作用机理,旨在为开发缓解疲劳和提高运动能力的系列天然产品提供可靠的实验室依据。方法:将50只wistar大鼠根据随机数字表法分为五组,每组各10只,分别为空白对照组(未给药未运动)、疲劳模型组(未给药的运动大鼠)、高剂量给药组[2.8 g/(kg·d)熊胆粉]、中剂量给药组[1.4 g/(kg·d)熊胆粉]、低剂量给药组[0.7 g/(kg·d)熊胆粉]。空白对照组和疲劳模型组大鼠每日给予相应剂量的蒸馏水。末次灌胃给予熊胆粉溶液后,将各组大鼠分别置于段氏实验跑台,观察其初始时刻状态,记录并对比各组大鼠跑台运动时间;取血,检测并对比各组大鼠血清血乳酸(BLA)、尿素氮(BUN)表达及乳酸脱氢酶(LDH)活力;取血后将大鼠处死,取大鼠肝脏,精密称取100 mg,另取各实验组大鼠后肢大腿内侧肌肉组织100 mg,检测并对比其肝糖原(LG)、肌糖原(MG)含量。结果:五组运动大鼠中,高剂量给药组运动时间>中剂量给药组>低剂量给药组>疲劳模型组,组间两两比较,差异均有统计学意义(P<0.05)。五组大鼠中,空白对照组血清BLA、BUN表达、LDH活力最低,后依次为高剂量给药组、中剂量给药组、低剂量给药组,疲劳模型组最高。血清BLA、BUN表达五组组间两两比较,差异均有统计学意义(P<0.05);中剂量给药组与高剂量给药组LDH活力比较,差异无统计学意义(P>0.05),其他组间两两比较差异均有统计学意义(P<0.05)。五组大鼠中,高剂量给药组大鼠LG、MG表达均最高,后依次为中剂量给药组、低剂量给药组,疲劳模型组表达均最低;各组MG含量组间两两比较差异均有统计学意义(P<0.05);中剂量给药组与低剂量给药组LG含量比较差异无统计学意义(P>0.05);其他组间两两比较差异均有统计学意义(P<0.05)。结论:熊胆粉能够提高运动性疲劳大鼠LDH活力,增加其LG与MG含量,使大鼠BLA含量降低,运动耐力大大增强,药物抗运动性疲劳效果理想。

Objective:To analyze the anti-fatigue effect of bear bile powder in rats with exercise-induced fatigue and its mechanism of action,and to provide reliable laboratory basis for the development of a range of natural products that relieve fatigue and improve athletic performance.Method:A total of 50 wistar rats were divided into 5 groups according to random number table,with 10 rats in each group,they were blank control group(without administration and exercise),fatigue model group(exercised rats were without administration),high-dose administration group(exercised rats were given 2.8 g/kg Bear Bile Powder),middle-dose administration group(exercised rats were given 1.4 g/kg Bear Bile Powder),low-dose administration group(exercised rats were given 0.7 g/kg Bear Bile Powder).The rats in blank control group and fatigue model group were given same dose of distilled water each day.After the last gavage with Bear Bile Powder solution,each group of rats was placed on the Duan’s experimental treadmill,the initial time state was observed,and the treadmill running time of each group of rats were recorded and compared.The blood of rats were taken,the blood lactic acid(BLA),blood urea nitrogen(BUN)expression and lactate dehydrogenase(LDH)activity in each group were detected and compared.After taking the blood,the rats were sacrificed and the liver of the rats were taken.100 mg inner thigh muscle tissue of the hind limbs of rats were taken,and the contents of liver glycogen(LG)and muscle glycogen(MG)were detected and compared.Result:Among five groups of exercised rats,the exercise time of high-dose administration group was longer than that of middle-dose administration group,low-dose administration group and fatigue model group,and the difference was statistically significant between two groups(P<0.05).Among five groups of rats,the serum BLA,BUN expression and LDH activity in blank control group were the lowest,followed by high-dose administration group,middle-dose administration group,and low-dose administration group,and fatigue model group was the highest.The statistical analysis found that there was statistical difference in the expression of serum BLA and BUN among five groups(P<0.05).There was no statistical difference in the LDH activity between middle-dose administration group and high-dose administration group(P>0.05),but there was statistical difference between other two groups(P<0.05).Among five groups of rats,the expression of LG and MG were the highest in the high-dose administration group,followed by the middle-dose administration group and low-dose administration group,and the expression of fatigue model group was the lowest.The statistical analysis found that there was significant difference in the content of MG between two groups(P<0.05);There was no statistical difference in the content of LG between middle-dose administration group and high-dose administration group(P>0.05),but there was statistical difference between other two groups(P<0.05).Conclusion:Bear Bile Powder for rats with exercise-induced fatigue can increase LDH activity,increase LG and MG contents,decrease BLA in rats,enhance exercise tolerance,and achieve ideal antifatigue effect.