lncRNA TUG1对高糖诱导的小鼠足细胞MPC5凋亡的影响

Effects of lncRNA TUG1 on high glucose-induced apoptosis of mouse podocyte MPC5

目的:探讨长链非编码RNA牛磺酸调节基因1(lncRNA TUG1)对高糖诱导的足细胞MPC5凋亡的影响。方法:小鼠足细胞MPC5分成对照组、高糖组、阴性对照高糖组、TUG1过表达高糖组,培养48 h后采用qRT-PCR检测TUG1表达变化,PI及Annexin V-FITC染色后采用流式细胞术测定细胞凋亡率,Western blot法检测细胞中Cleaved-Caspase-12、Cleaved-PARP、CHOP、p-eIF2α、GRP78蛋白表达水平。结果:与对照组比较,高糖组细胞中TUG1表达水平降低,细胞凋亡率和Cleaved-Caspase-12、Cleaved-PARP、CHOP、p-eIF2α、GRP78蛋白表达水平增加(P<0.05)。与阴性对照高糖组比较,TUG1过表达高糖组细胞中TUG1表达水平升高,细胞凋亡率和Cleaved-Caspase-12、Cleaved-PARP、CHOP、p-eIF2α、GRP78蛋白表达水平下降(P<0.05)。结论:lncRNA TUG1可抑制高糖诱导的足细胞MPC5凋亡,作用机制可能与降低内质网应激有关。

Aim:To investigate the effects of long non-coding RNA taurine up-regulated gene 1(lncRNA TUG1,on apoptosis of podocytes induced by high glucose in mouse podocyte MPC5.Methods:Mouse podocyte MPC5 was divided into control group,high glucose group,high glucose culture group(high glucose+lentivirus infection with negative control),TUG1+high glucose group(high glucose+TUG1 overexpression lentivirus infection).After being cultured for 48 hours,qRT-PCR was used to detect the expression of TUG1;flow cytometry was used to detect cell apoptosis after PI and Annexin V-FITC staining;Western blot was used to detect the expressions of Cleaved-Caspase-12,Cleaved-PARP,CHOP,p-eIF2αand GRP78 proteins in cells.Results:Compared with control group,the expression of TUG1 was decreased in the high glucose group,and the apoptosis rate and expressions of Cleaved-Caspase-12,Cleaved-PARP,CHOP,p-eIF2αand GRP78 proteins were increased(P<0.05).Compared with high glucose culture group,the expression of TUG1 was increased in the TUG1+high glucose group,the apoptosis rate and expressions of Cleaved-Caspase-12,Cleaved-PARP,CHOP,p-eIF2αand GRP78 proteins were decreased(P<0.05).Conclusion:lncRNA TUG1 could inhibit apoptosis of podocytes MPC5 induced by high glucose,and the mechanism may be related to the reduction of endoplasmic reticulum stress.