HOXA6基因过表达对肺癌A549细胞增殖和凋亡的影响

Effects of HOXA6 gene overexpression on proliferation and apoptosis of lung cancer A549 cells

目的:探讨HOXA6基因过表达对肺癌A549细胞增殖和凋亡的影响。方法:采用qRT-PCR和Western blot法检测正常支气管上皮BEAS-2B细胞和A549细胞中HOXA6 mRNA和蛋白的表达。将A549细胞分为正常对照组、阴性对照组(感染阴性对照慢病毒)和HOXA6过表达组(感染HOXA6过表达慢病毒),采用qRT-PCR法检测HOXA6 mRNA的表达,CCK-8法检测细胞增殖情况,用Annexin V-APC/7-AAD法检测细胞凋亡情况,Western blot法检测HOXA6、cleaved Caspase-3、cleaved Caspase-9、Bax和Bcl-2蛋白的表达。结果:与BEAS-2B细胞相比,A549细胞中HOXA6 mRNA和蛋白的表达均下降(P<0.05);与阴性对照组相比,HOXA6过表达组A549细胞中HOXA6 mRNA和蛋白的表达增加,细胞存活减少,细胞凋亡增加,cleaved Caspase-3和cleaved Caspase-9蛋白的表达升高,Bax/Bcl-2比值增加(P均<0.05)。结论:HOXA6可能通过Bcl-2相关通路诱导肺癌细胞凋亡,抑制其增殖。

Aim:To explore the effects of HOXA6 overexpression on cell proliferation and apoptosis of A549 cells.Methods:The mRNA and protein levels of HOXA6 in normal bronchial epithelial BEAS-2B cells and A549 cells were detected by qRT-PCR and Western blot,respectively.A549 cells were divided into normal control group,negative control group(infected with negative control lentivirus)and HOXA6 overexpression group(infected with HOXA6 overexpression lentivirus).The expression of HOXA6 mRNA was detected by qRT-PCR.Cell survival rate was detected by CCK-8 method.Cell apoptosis was measured by Annexin V-APC/7-AAD staining.The expressions of HOXA6,cleaved Caspase-3,cleaved Caspase-9,Bax and Bcl-2 proteins were detected by Western blot.Results:Compared with BEAS-2B cells,the mRNA and protein expressions of HOXA6 in A549 cells were decreased(P<0.05).Compared with negative control group,the expressions of HOXA6 mRNA and protein were increased,cell survival rate was decreased,the number of apoptotic cells was increased,the expressions of cleaved Caspase-3 and cleaved Caspase-9 were increased,and the ratio of Bax/Bcl-2 was increased in the HOXA6 overexpression group(P<0.05).Conclusion:HOXA6 can induce apoptosis and inhibit proliferation of lung cancer cells through Bcl-2 related pathway.