葡萄F1代杂交种的SRAP分子标记鉴定

Identification of F1 hybrids of grapes by SRAP molecular marker

葡萄在遗传上高度杂合,从分子水平上对葡萄杂交后代的真伪性进行早期、快速鉴定,旨在为加速葡萄种质创制提供辅助手段。采用SRAP分子标记技术对3对葡萄杂交组合红提×红玫瑰、红提×贝达和红提×贵妃的8个F1代实生苗进行了杂种真实性鉴定。从196对SRAP-PCR引物中筛选出10对可以在红提与贝达间产生清晰、稳定的多态性条带的引物,其中有4对引物能够区别红提与红玫瑰,有4对引物能够区别红提与贵妃;各选取3对引物分别对3对杂交组合的F1代实生苗进行鉴定。结果表明:8个F1代实生苗均为真杂种。该研究为利用SRAP标记技术有效、快速鉴定葡萄杂种后代提供依据。

Grape is genetically highly heterozygous.An early and rapid identification of the authenticity of grape hybrid progenies at the molecular level provides an auxiliary means for accelerating the creation of grape germplasm.SRAP molecular marker technique was used to identify the authenticity of eight F1 seedlings of three hybrid combinations of grape,‘Hongti’בRed Rose’,‘Hongti’בBeda’and‘Hongti’בGuifei’.Ten pairs of primers selected from 196 pairs of SRAP-PCR primers could produce clear and stable polymorphism bands between Hongti and Beda.Among them,four pairs of primers could distinguish between‘Hongti’and‘Red rose’grape,and 4 pairs of primers between‘Hongti’and‘Guifei’grape.Three pairs of primers were selected respectively to identify the F1 seedlings of three hybrid combinations,and the results showed that all the eight F1 seedlings were true hybrids.This study provides a basis for the effective and rapid identification of hybrid progenies using SRAP markers.