葱兰愈伤组织诱导及植株再生

Callus induction and plant regeneration of Zephyranthes candida

为了给园林绿化提供更多的优质种苗,以葱兰种子作为外植体进行组织培养。结果表明:低浓度的2,4-D单独使用对葱兰种子愈伤组织的诱导效果不明显,MS+2,4-D 0.2 mg/L处理葱兰种子愈伤组织诱导率仅为3.3%;复合使用6-BA后葱兰种子愈伤组织和丛生芽的诱导率明显增加,MS+2,4-D 0.4 mg/L+6-BA 0.2 mg/L处理葱兰种子愈伤组织诱导率为90.0%,MS+6-BA 1.0 mg/L+NAA 1.0 mg/L处理葱兰丛生芽诱导率为66.7%。结论为诱导葱兰种子愈伤组织最佳培养基是MS+2,4-D 0.4 mg/L+6-BA 0.2 mg/L,诱导葱兰丛生芽的最佳培养基是MS+6-BA 1.0 mg/L+NAA 1.0 mg/L。

The seeds of Zephyranthes candida as the explants were cultured in vitro for providing a higher quality seedling to landscaping.The results showed that the single addition of a low concentration of 2,4-D to the medium was ineffective to induce callus,the callus induction rate was only 3.3%when treated with MS+2,4-d 0.2mg/L,while 2,4-D together with 6-BA in the medium was promoting induction of callus and multiple shoots obviously.The callus induction rate was 90%on medium of MS containing 0.4 mg/L 2,4-D and 0.2 mg/L 6-BA,while the multiple shoot induction rate was 66.7%on medium of MS containing 1 mg/L 6-BA and 1 mg/L NAA.The callus induction optimal media was MS+2,4-D 0.4 mg/L+6-BA 0.2 mg/L,and the multiple shoot induction optimal media was MS+6-BA 1 mg/L+NAA 1 mg/L.