本草香对主流烟气暴露大鼠心血管系统蛋白组学的影响研究

Differential protein expressions in heart tissues from rats exposed to cigarette smoke

目的:比较添加本草香成分香烟与普通香烟暴露后大鼠心脏组织中的差异表达蛋白及其涉及的生物学通路。方法:将24只SD大鼠分为2组:普通香烟烟气暴露组和含本草香成分香烟(金圣4)烟气暴露组,经过3个月主流烟气暴露后取心脏组织,采用串联质谱标记(TMT)定量蛋白质组学研究技术对两组大鼠心脏组织差异表达蛋白进行筛选,利用基因本体(GO)和京都基因与基因组百科全书(KEGG)数据库对差异蛋白进行生物信息学分析。结果:筛选出金圣4香烟组大鼠和普通香烟烟气暴露组大鼠心脏组织差异表达1.2倍以上的蛋白43个,差异表达蛋白GO分析结果发现,这些蛋白涉及分子功能、细胞组分及生物学过程3个分类;对金圣4香烟组大鼠心脏组织差异表达蛋白进行KEGG富集分析发现,差异表达蛋白涉及6个生物学通路。结论:本研究寻找到本草香成分香烟与普通香烟暴露后大鼠心脏组织中差异表达蛋白及差异蛋白所涉及的生物学通路,可以为进一步研究本草香的生物学功效提供帮助和研究线索。

OBJECTIVE:To compare biological pathways involved in the differential expression of proteins and differential proteins in heart tissues from rats after their exposure to normal and herbal cigarette smoke.METHODS:Twenty-four Sprague-Dawley rats were divided into two groups:1)normal cigarette smoke exposure and 2)herbal-containing cigarette smoke exposure(Jinsheng 4).The tandem mass tags(TMT)quantitative proteomics assay was used.After 3 months of smoke exposure,the differentially expressed proteins in the heart tissues from the two groups were screened.The gene ontology(GO)and the Kyoto Gene and Genomic Encyclopedia(KEGG)database were used to identify the differential proteins and bioinformatics analyses were conducted.RESULTS:A total of 43 proteins with different expressions of more than 1.2-fold were identified.The GO analyses of differentially expressed proteins indicate that these proteins were involved with molecular functions,cellular components and biological processes.KEGG enrichment analyses of differentially expressed proteins in the Jinsheng 4 group show that differentially expressed proteins were involved in six biological pathways.CONCLUSION:This study identifies differential expression of proteins,differential proteins and their biological pathways in heart tissues from rats after their exposure to herbal and common cigarette smoke.Our study provides clues for harm reduction among cigarette smokers.