甲基丙烯酸环氧丙酯的遗传毒性评价

Genotoxicity evaluation of glycidyl methacrylate

目的:观察甲基丙烯酸环氧丙酯(GMA)诱发体外培养的中国仓鼠肺细胞(V79)微核发生变化情况,对其遗传毒性进行评价。方法:分别采用不同浓度(2.25、4.5、9.0、18.0、36.0μg/mL)的GMA染毒V79细胞,设置空白对照组和DMSO溶剂对照组,其中染毒3 h处理组分别在加和不加体外活化系统(S9)条件下进行,染毒24 h处理组在不加S9条件下进行。分别计算细胞复制指数和微核细胞率。结果:GMA的浓度在2.25~36.0μg/mL范围内,无S9的条件下,与空白对照组和DMSO溶剂对照组相比,GMA染毒3和24 h组的V79细胞复制指数均无明显变化,但微核细胞率明显升高,并呈浓度-效应关系;在有S9的条件下染毒3 h,各剂量组的GMA诱发微核细胞率的差异均无统计学意义。结论:在2.25~36.0μg/mL浓度范围,GMA可致V79细胞的微核率升高,表明GMA可诱发遗传物质损伤,具有遗传毒性。

OBJECTIVE:To evaluate genotoxicity of glycidyl methacrylate(GMA)in Chinese hamster lung cells(V79)using the micronucleus assay.METHODS:V79 cells were treated with different doses of GMA(2.25,4.5,9.0,18.0,36.0μg/mL)for 3 h with or without an in vitro activation system(S9).Controls include a blank and a DMSO group.Another treatment group was treated for 24 h without S9.Cell replication indices and binuclear micronucleus rates were determined.RESULTS:Without S9,the replication index of the treated V79 cells did not change significantly but the incidence of micronuclei in binuclear cells increased significantly in a dose-dependent manner.With the addition of S9,the rate of micronucleated cells in the high-dose group was not statistically significant.CONCLUSION:In the concentration range of 2.25-36.0μg/mL,G MA induced micronuclei in V79 cells,indicating that GMA is genotoxic.