滋生青苔对柑橘叶际生物多样性的影响

Influence of moss disease on the biodiversity of phyllosphere microbe on citrus leaves

本文利用Illumina Miseq高通量测序技术对重庆地区有青苔病症和无病症的柑橘叶际真核生物的群落组成、结构、多样性及差异进行了研究,旨在找到可能的病原物类群,为更好地防治柑橘青苔病奠定基础。结果表明13个柑橘叶片样本共检测到303个OTU,多样性较为丰富;优势门为链形植物门Streptophyta、子囊菌门Ascomycota、绿藻门Chlorophyta等;通过分析发现可能的病原物为不可培养的虚幻球藻属球藻uncultured Apatococcus sp.、黄绿异小球藻Heterochlorella luteoviridis、无柄杯梗孢Cyphellophora sessilis、海南橡胶藻Heveochlorella hainangensis、Coniochaetales sp. GMG C4、椭圆球藻Chloroidium ellipsoideum、Kalinella bambusicola等;不同区县表现出青苔病症状的柑橘叶片上疑似病原物的种类和丰度都差异很大。柑橘青苔病可能是由多种藻类及真菌复合侵染造成的,病原物和柑橘叶片间的相互作用有待进一步研究。

The eukaryotic diversity of citrus phyllosphere microbe communities after moss infection in Chongqing was analyzed to find the possible pathogens, and to lay the foundation for better controlling citrus moss disease. In this paper, Illumina Miseq high-throughput sequencing technology was used to explore the differences of eukaryotic composition and structure community between having symptoms samples and no symptoms samples in Chongqing. 303 OTU were detected from 13 citrus leaves samples, and the diversity of samples was abundant. The results showed that the advantage phylum were Streptophyta, Ascomycota, and Chlorophyta. The advantage classes were Dothideomycetes, Trebouxiophyceae, Eurotiomycetes, Sordariomycetes. The possible pathogens were uncultured Apatococcus sp., Heterochlorella luteoviridis, Cyphellophora sessilis, Heveochlorella hainangensis, Coniochaetales sp. GMG C4, Chloroidium ellipsoideum and Kalinella bambusicola etc. The species and abundance of suspected pathogens varied widely. The occurrence of citrus moss disease may be caused by a variety of algae and fungal complex infection, the interaction between pathogen and citrus leaves remains to be further studied.