多抗霉素B在鲜人参中的储藏稳定性

Storage stability of polyoxins B in fresh Panax ginseng

建立了鲜人参样品中多抗霉素B的简易样品前处理程序和HPLC-MS/MS检测方法,对0~150 d低温储藏的鲜人参添加样品进行了分析,考察了多抗霉素B在鲜人参样品中的低温储藏稳定性。结果表明,所建立分析方法在0.01~2.0 mg/L范围内线性响应良好,0.2~5.0 mg/kg添加水平的平均回收率为78.0%~94.7%,定量检测限为0.275 mg/kg;鲜人参样品在-18℃和-20℃储藏条件下多抗霉素B的平均降解率均小于30%,说明多抗霉素B在鲜人参样品中至少可以稳定储藏150 d。该结果为多抗霉素B在人参上的残留登记试验提供了可靠的科学依据。

In order to investigate the low temperature storage stability of polyoxins B in fresh Panax ginseng samples, the spiked fresh P.ginseng which were stored at-18℃ and-20℃ for 0-150 days were analyzed by the established simple pretreatment program and high performance liquid chromatography tandem mass spectrometry detection method. The results showed that the established method had a good linear response in the range of 0.01-2.0 mg/L, the average recovery of polyoxins B spiked at 0.2-5.0 mg/kg in blank P.ginseng was between 78.0% and 94.7%, and the limit of quantitative detection was 0.275 mg/kg. The average degradation rate of polyoxins B in fresh P.ginseng samples was less than 30% at-18℃ and-20℃, indicating that polyoxins B can be steadily stored for at least 150 days in fresh P.ginseng samples. This result provides a reliable scientific basis for the polyoxins B residue field trials on P.ginseng field.