B淋巴细胞刺激因子在妊娠期高血压疾病中的动态变化

Dynamic changes of B-lymphocyte stimulator in hypertensive disorder complicating pregnancy

目的通过动态监测孕妇不同时期血清B淋巴细胞刺激因子(BAFF)的水平,了解其在妊娠期高血压疾病(HDCP)发生、发展中的作用。方法于孕前、孕10~12周、孕24~26周及孕34~36周时检测孕妇血压并采集外周血保存,根据分娩时情况选择妊娠期高血压(GH)、子痫前期(PE)、正常妊娠组产妇各30例,以双抗体两步夹心酶联免疫吸附试验测定孕妇4个时间节点的血清BAFF。结果PE组发现高血压的时间及终止妊娠的时间均早于GH组(P<0.05),且PE组孕妇的平均动脉压(MAP)大于GH组和正常妊娠组(P<0.05)。GH组和正常妊娠组相比,孕妇终止妊娠时间和分娩前MAP也差异有统计学意义(P<0.05);3组产妇在非孕期BAFF值比较差异无统计学意义(P>0.05),但在孕早期、孕中期及孕晚期,PE组血清BAFF值均高于GH组(P<0.05)和正常妊娠组(P<0.05),而GH组血清BAFF值高于正常妊娠组(P<0.05)。3组产妇的血清BAFF值均随妊娠进展逐渐下降(P<0.05);非孕期产妇血清BAFF值与分娩前MAP相关性差异无统计学意义(P>0.05),但孕早期、孕中期及孕晚期血清BAFF值与分娩前MAP均有明显相关(r=0.589、0.571、0.684,P<0.01)。结论BAFF在正常妊娠的开始和维持及HDCP的发生、发展中都起着重要作用。

Objective To investigate the role of serum B lymphocyte stimulating factor(BAFF)in the occurrence and development of hypertensive disorder complicating pregnancy(HDCP)by dynamically monitoring the serum BAFF level in different periods.Methods The blood pressure was measured and peripheral blood was collected for conservation before pregnancy,in 10-12 weeks,24-26 weeks and 34-36 weeks of gestation.Each 30 cases of gestational hypertension(GH),preeclampsia(PE)and normal pregnancy were randomly selected according to the delivery situation.Serum BAFF levels at 4 time nodes of pregnant women were determined by using the double antibody sandwich enzyme-linked immunosorbent assay.Results The time of found hypertension and pregnancy termination in the PE group was earlier than that in the GH group(P<0.05),moreover the mean arterial pressure(MAP)in the PE group was greater than that in the GH group and normal group(P<0.05).The termination time and MAP before delivery had the statistical difference between the GH group and normal pregnancy group(P<0.05);there was no statistically significant difference in BAFF value during the non-pregnancy period among the three groups(P>0.05),but the BAFF values during the early pregnancy,middle pregnancy and late pregnancy in the PE group were higher than those in the GH group(P<0.05)and the normal pregnancy group(P<0.05),while the srum BAFF value in the GH group was higher than that in the normal pregnancy group(P<0.05).The serum BAFF values of the three groups were decreased gradually with the pregnancy development(P<0.05);there was no statistically significant difference in the correlation between the BAFF value of non-pregnant women and the pre-delivery MAP(P>0.05);however,the serum BAFF values in early pregnancy,middle pregnancy and late pregnancy all had the significant correlation with MAP before delivery(r=0.589,0.571,0.684,P<0.05).Conclusion BAFF plays the important role in the beginning and maintenance of normal pregnancy and the occurrence and development of HDCP.