氨基酸/羟基磷灰石复合材料应用于酸蚀牛牙釉质体外再矿化（英文）

In Vitro Remineralization of Acid-etched Bovine Enamel with Amino Acids/Hydroxyapatite Composite

早期牙釉质龋病的再矿化在龋齿的预防和修复中起着至关重要的作用。基于牙釉质基质中所含的主要氨基酸,在10 mmol/L甘氨酸、10 mmol/L L-丝氨酸和5 mmol/L L-天冬氨酸的存在下制备了氨基酸/羟基磷灰石(AA/HAP)复合材料,表征了其物理、化学和生物学性质,并评估了其对酸蚀牛牙釉质的再矿化作用。与不含氨基酸的羟基磷灰石(HAP)相比,在氨基酸的抑制作用下,AA/HAP复合材料具有更低的结晶度和更高的生物相容性。在人工唾液中用AA/HAP对酸蚀牛牙釉质进行体外再矿化。再矿化后,分别表征了牛牙釉质样品的表面和横截面形态、成分和力学性能。结果表明, AA/HAP可以诱导表面和深层牙釉质病变的修复。复合材料中释放的氨基酸可以吸附在有机基质残基上并诱导平行排列的HAP晶体的形成,从而使牙釉质表面显微硬度(SMH)得到显著恢复。最后,讨论了AA/HAP复合材料对酸蚀牛牙釉质的再矿化机制。

Remineralization of early enamel caries lesions plays a crucial role in prevention and restoration of dental caries. Based on the crucial amino acids contained in enamel matrix, amino acids/hydroxyapatite(AA/HAP) composite was synthesized in the presence of 10 mmol/L glycine, 10 mmol/L L-serine and 5 mmol/L L-aspartic acid. The physical, chemical and biological properties of the composite were characterized. And its remineralization effect on acid-etched bovine enamel was evaluated. Under the inhibition properties of amino acids, the composite has lower crystallinity and higher biocompatibility compared to the hydroxyapatite(HAP) without amino acids. In vitro remineralization of acid-etched bovine enamel using AA/HAP was conducted in artificial saliva. After remineralization, the surface and cross-section morphology, the components, and the mechanical property of bovine enamel samples were characterized, respectively. The results showed that the AA/HAP could induce the restoration of both surface and subsurface enamel lesions. The amino acids released from the composite could adsorb on the organic matrix residues and induce the formation of parallel arranged HAP crystals, eventually formed significant surface microhardness(SMH) recovering.