蛴螬提取物对脉络膜新生血管中趋化因子受体3及其配体嗜酸性粒细胞趋化因子表达的影响

Effects of Grub Extract on Expression of Chemokine Receptor 3 and Its Ligand Eotaxin in Choroidal Neovascularization

目的观察蛴螬提取物口服、滴眼两种给药途径对实验眼脉络膜新生血管(Choroidal neovascularization,CNV)组织的趋化因子受体3(chemokine receptor 3,CCR3)及其配体嗜酸性粒细胞趋化因子(eosinophil activated chemotactic factor,Eotaxin)表达的影响。方法选用8周龄雄性BN大鼠32只,随机数字表法平均分成空白组、模型组、口服组、滴眼组,予以不同干预措施。采用YAG激光建立CNV大鼠模型,于造模后7、28 d行荧光素眼底血管造影(fluorescein fundus angiography,FFA)检查,造模后28 d行免疫组化CCR3及其配体Eotaxin染色及其结果观察分析。结果FFA检查显示:造模后7 d除空白组外,各组均造模成功;造模后28 d口服组与滴眼液荧光渗漏减少。CCR3及Eotaxin免疫组化结果示:口服组与滴眼组CCR3及Eotaxin含量较模型组减少,差异有统计学意义(P<0.05);口服组与滴眼组相比,差异无统计学意义(P>0.05)。结论蛴螬提取物两种给药途径均可抑制实验性CNV模型中CCR3及其配体Eotaxin的表达,从而抑制实验性CNV的形成。

Objective To observe the effects of grub extract on chemokine receptor 3(CCR3)and its ligand eosinophil activated chemotactic factor(Eotaxin)in the choroidal neovascularization(CNV)tissue of the experimental ocular.Methods A total of 32 male BN rats of 8 weeks old were randomly divided into a blank group,a model group,an oral administration group and an eye drop group by random number table method,and different intervention measures were given.The rat model of CNV was established by YAG laser.Fluorescein fundus angiography(FFA)was performed 7 days and 28 days after model establishment.Immunohistochemical staining of CCR3 and its ligand Eotaxin was observed 28 days after model establishment and its results were observed and analyzed.Results The FFA examination showed that all the groups were successful in modeling except the blank group 7 d after the model establishment;the fluorescence leakage of the oral administration group and the eye drop group was reduced 28 days after model establishment.The results of CCR3 and Eotaxin immunohistochemistry showed that the levels of CCR3 and Eotaxin in the oral administration group and the eye drop group were significantly decreased than those in the model group,with statistically significant differences(P<0.05).The difference between the oral administration group and the eye drop group was not statistically significant(P>0.05).Conclusion Both routes of sputum extract can inhibit the expression of CCR3 and its ligand Eotaxin in the experimental CNV model,thereby inhibiting the formation of experimental CNV.