摘要
探讨丝切蛋白1(Cofilin-1)与肝癌细胞的迁移、侵袭能力的关系,运用免疫印迹试验(western blot)检测肝癌细胞(Huh-7细胞)、转染上皮生长因子受体的人肝癌细胞(Huh-7-EGFR细胞)与转染上皮生长因子受体突变体Ⅲ的人肝癌细胞(Huh-7-EGFRvⅢ细胞)三种细胞中Cofilin-1的蛋白表达。然后将Cofilin-1用慢病毒表达系统导入Huh-7细胞,构建转染丝切蛋白1的人肝癌细胞(Huh-7-Cofilin-1细胞)系,并应用迁移侵袭实验检测细胞迁移侵袭的能力;再用RNA干扰(siRNA)技术沉默Huh-7-EGFRvⅢ细胞中的Cofilin-1,然后应用迁移侵袭实验检测细胞的迁移侵袭能力。结果显示:转染Cofilin-1后,Huh-7细胞的迁移侵袭能力显著增强(P<0.05);而干扰Cofilin-1后,Huh-7-EGFRvⅢ细胞迁移与侵袭能力明显减弱(P<0.05)。表明Cofilin-1参与肝癌细胞迁移与侵袭。
In order to study the relationship between cofilin-1 and the migration and invasion ability of hepatocellular carcinoma cells.The protein expression of Cofilin-1 in Huh-7 cells and Huh-7-EGFR cells and Huh-7-EGFRv Ⅲ cells were detected by western blot.Cofilin-1 was transfected into Huh-7 cells by lentivirus expression system.Cofilin-1 was silenced in the Huh-7-EGFRv Ⅲ cells.The migration or invasion ability of cells were analyzed and verified by transwell migration assay and matrigel invasion assay.The protein expression of Cofilin-1 in Huh-7-EGFRv Ⅲ cells was significantly higher than that in Huh-7 and Huh-7-EGFR cells.After transfection of Cofilin-1,the migration and invasion ability of Huh-7 cells were enhanced.Conversely,after interference Cofilin-1,the migration and invasion ability of Huh-7-EGFRv Ⅲ cells were decreased.In conclusion,the migration and invasion of Hepatocellular Carcinoma cells were promoted by Cofilin-1.
作者
龙晓兰
张万勇
吴勇军
刘德勇
LONG Xiaolan;ZHANG Wanyong;WU Yongjun;LIU Deyong(Department of Pathology,the First People's Hospital of Xiangtan City,Xiangtan 400110,Hunan,China;Hunan University of Environment and Biology,Hengyang 421001,Hunan,China)
出处
《中南医学科学杂志》
CAS
2019年第6期571-575,600,共6页
Medical Science Journal of Central South China
基金
湖南省教育厅高校科研课题(12C1051)
衡阳市科学技术局课题(2012KJ23)
关键词
Cofilin-1
肝癌
迁移
侵袭
Cofilin-1
hepatocellular carcinoma
migration
invasion