HPLC测定不同地区桑椹饮片中七种成分含量

Simultaneous Determination of Seven Components in Mori Fructus Slice from Different Areas of China by HPLC

目的:建立同时测定桑椹饮片中新绿原酸、绿原酸、隐绿原酸、芦丁、异槲皮苷、紫云英苷和槲皮素7种成分含量的高效液相色谱(HPLC)测定方法,为评价中国不同地区桑椹饮片的质量提供技术手段。方法:采用ODS-C18(250 mm×4.6 mm,5μm)色谱柱,水(含0.2%甲酸)-乙腈为流动相,梯度洗脱,流速为1 mL·min-1,检测波长为360 nm。结果:新绿原酸、绿原酸、隐绿原酸、芦丁、异槲皮苷、紫云英苷和槲皮素7种成分线性关系良好,加样回收率在95.0%~105%。不同地区使用的桑椹饮片中7种成分含量差异较大。结论:本研究建立了一种稳定、可靠的桑椹饮片中7种成分含量同时测定的高效液相色谱方法,为桑椹饮片质量研究提供参考。

Objective:To establish an HPLC-UV method for simultaneous determination of neochlorogenic acid,chlorogenic acid,cryptochlorogenic acid,rutin,quercetin,astragaline and quercetin for quality evaluation of Mori Fructus slice.Methods:The analysis was carried out on an ODS-2 column(250 mm×4.6 mm,5μm)column with water(containing 0.2%formic acid)-acetonitrile as the mobile phase by RP-HPLC method.The detection wavelength was 360 nm and flow rate was 1 mL·min-1.Results:Seven components showed respectively a good linear relationship.The recoveries were 95.0%~105%.The contents of seven components in Mori Fructus slice from different areas was quite different.Conclusion:The simple and accurate HPLC-UV method was established and validated to determine the contents of seven components for quality evaluation of Mori Fructus.