老鸦瓣黄酮的制备及其稳定性研究

Preparation and Stability Characterization of Flavonoids from Tulipa edulis

以老鸦瓣叶片为材料,65%乙醇为提取剂,D-110大孔树脂纯化制备老鸦瓣黄酮,比色法测定其黄酮含量,红外光谱扫描观察其基团特征,并考察温度、pH、氧化剂、还原剂、光照等对其稳定性的影响。结果表明,制备得到的老鸦瓣黄酮为槲皮素、芦丁等黄酮类化合物,具有良好的醇溶性和水溶性等特点。老鸦瓣黄酮在低于85℃、自然光或避光以及偏酸性环境下较为稳定,氧化剂H2O2对其稳定性影响也不显著(P>0.05),但还原剂Na2SO3对其稳定性影响显著(P<0.05)。因此,在试验范围内,除还原剂Na2SO3存在之外,老鸦瓣黄酮的性质较为稳定,且具有醇溶性和水溶性特点,适合开发与利用。

Flavonoids from leaves of Tulipa edulis were prepared with D-110 macroporous resin purification and 65%ethanol as extractant.The contents of flavonoids were determined by colorimetry.Their group characteristics were observed by infrared spectroscopy.The effects of temperature,pH,oxidant,reductant,illumination on their stability were investigated.The results showed that flavonoids from Tulipa edulis contained quercetin,rutin and other flavonoids,which had good alcohol solubility and water solubility.Flavonoids from Tulipa edulis were stable under the conditions of less than 85℃,natural light or shelter from light and acidic environment.The effect of oxidant H2 O2 on their stability was not significant(P>0.05).However,reductant Na2 SO3 had a significant effect on their stability(P<0.05).Therefore,in the test range,except for reducing agent Na2 SO3,the flavonoids from Tulipa edulis were stable.They have good alcohol solubility and water solubility,which are suitable for development and utilization.