miR-367对人乳腺癌MCF-7细胞增殖、侵袭和迁移的影响及其机制

Effects of miR-367 on proliferation,invasion and migration of human breast cancer MCF-7 cells and their mechanisms

目的:探讨miR-367对人乳腺癌MCF-7细胞增殖、侵袭和迁移的影响,并阐明其作用机制。方法:检测乳腺癌组织、癌旁组织、MCF-7细胞和MCF-10A细胞中miR-367相对表达水平。将miR-NC或miR-367-inhibitor转入MCF-7细胞作为阴性对照组和miR-367-inhibitor组,检测2组MCF-7细胞活性、细胞集落数、Ki67阳性表达率、细胞划痕愈合率、侵袭细胞数和KLF4相对表达水平。将miR-NC或miR-367-mimic转入MCF-7细胞作为阴性对照组和miR-367-mimic组,检测2组MCF-7细胞集落数、细胞划痕愈合率、侵袭细胞数和KLF4相对表达水平。结果:与癌旁组织或MCF-10A细胞比较,乳腺肿瘤组织和MCF-7细胞中miR-367相对表达水平均明显升高(P<0.01)。与阴性对照组比较,miR-367-inhibitor组MCF-7细胞中miR-367相对表达水平、细胞活性、细胞集落数、Ki67阳性表达率、细胞划痕愈合率和侵袭细胞数均明显降低(P<0.01),KLF4相对表达水平明显升高(P<0.01);与阳性对照组比较,miR-367-mimic组MCF-7细胞中miR-367相对表达水平、细胞集落数、细胞划痕愈合率和侵袭细胞数均明显升高(P<0.01),KLF4相对表达水平明显降低(P<0.01)。结论:miR-367可促进MCF-7细胞增殖、侵袭和迁移,其机制可能与抑制KLF4的表达有关。

Objective:To investigate the effects of miR-367 on theProliferation,invasion and migration of the human breast cancer MCF-7 cells,and to elucidate their mechanisms.Methods:The relative expression levels of miR-367 in the breast cancer tissues,adjacent tissues,MCF-7 cells and MCF-10A cells were detected.The MCF-7 cells were transfected with miR-NC or miR-367-inhibitor as negative control group and miR-367-inhibitor group,and the cell viabilities,the number of colonies,thePositive expression rates of Ki67,the rate of cell wound healing,the number of invasive cells and the relaitive expression levels of KLF4 in the MCF-7 cells in two groups were detected.The MCF-7 cells were transfected with miR-NC or miR-367-mimic as negative control group and miR-367-mimic group,and the number of colonies,the rates of cell wound healing,the number of invasive cells and the relaitive expression levels of KLF4 in the MCF-7 cells in two groups were detected.Results:Compared with adjacent tissue or MCF-10A cells,the relative expression levels of miR-367 in breast cancer tissues and MCF-7 cells were significantly increased(P<0.01).Compared with negative control group,the relative expression level of miR-367 in the MCF-7 cells,the cell viability,the number of colonies,thePositive expression rate of Ki67,the rate of cell wound healing and the number of invasive cells in miR-367-inhibitor group were significantly decreased(P<0.01),and the relative expression level of KLF4 was significantly increased(P<0.01);compared withPositive control group,the relative expression level of miR-367 in the MCF-7 cells,the number of colonies,rates of cell wound healing and number of invasive cells in miR-367-mimic group were significantly increased(P<0.01),and the relative expression level of KLF4 was significantly decreased(P<0.01).Conclusion:miR-367Promotes theProliferation,migration and invasion of MCF-7 cells,and the mechanism may be related to the inhibition of KLF4 expression.