钛表面氧化石墨烯涂层对施旺氏细胞黏附与增殖的影响

Graphene Oxide Modified Titanium Promote Adhesion and Proliferation of Schwann Cells

目的:研究纯钛表面氧化石墨烯修饰对施旺氏细胞黏附与增殖的影响。方法 :在纯钛片表面制备氧化石墨烯涂层,检测复合材料的表面形貌、接触角等特征。以未处理的光滑钛片和喷砂酸蚀后的钛片为对照组,分别以0.25、0.50 mg/mL氧化石墨烯溶液制备的钛片为实验组,将大鼠施旺氏细胞接种于材料表面。通过CCK8实验检测不同处理组钛片表面施旺氏细胞的增殖水平,活/死细胞染色检测材料的细胞毒性,鬼笔环肽和DAPI染色、扫描电镜观察施旺氏细胞接种24 h后的表面黏附形态,Real-time PCR检测施旺氏细胞中神经生长因子相关基因的表达水平。结果:氧化石墨烯涂层修饰后材料表面呈现典型的皱褶样结构。细胞培养第3、5天,实验组施旺氏细胞的增殖能力高于对照组,以0.25 mg/mL氧化石墨烯溶液涂层组最为显著。实验组材料表面,施旺氏细胞的胞体伸展较对照组更加充分,有多个细胞突起。结论:氧化石墨烯修饰的钛表面可以促进施旺氏细胞的黏附与增殖。

Objective: To explore the effects of graphene oxide modified titanium on the adhesion and proliferation of Schwann cells. Methods: Graphene oxide coating was prepared on the surface of pure titanium sheets by spin-coating method. The surface morphology and hydrophilic of the materials were examined. The untreated smooth titanium sheets and the sandblasted titanium sheets were used as control groups, the titanium sheets coating prepared through 0.25 mg/mL and 0.50 mg/mL graphene oxide solution were used as the experimental groups. Schwann cells were inoculated on the surface of the materials. The proliferation of Schwann cells on the surface of titanium slices was observed by CCK8 assay. The live/dead cell staining was used to observe the cytotoxicity of these materials. Phalloidin and DAPI staining was used to observe the adhesion morphology of Schwann cells after 24 hours of inoculation. Real-time PCR was used to detect the expression of neurofactors related genes in Schwann cells. Results: The graphene oxide modified surfaces showed a typical wrinkle-like structure. Compared with the control groups, the experimental groups could apparently promote the proliferation of Schwann cells, and the coating group with 0.25 mg/mL graphene oxide solution showed the most significant. The Schwann cells in the experimental groups were more fully stretched and had more processes than those in the control groups. Conclusion: Graphene oxide modified titanium surface can promote the adhesion and proliferation of Schwann cells.