用冻干菌液进行猪丹毒、多杀性巴氏杆菌病二联灭活疫苗效力检验的研究

Study on the Efficacy Test of Freeze-dried Strain for Combined Inactivated Swine Erysipelas and Pasteurella multocida Vaccine

在猪丹毒、多杀性巴氏杆菌病二联灭活疫苗的效力检验中,需要进行动物攻毒保护试验。传统方法使用的是2~8℃保存的新鲜菌液对试验动物进行攻毒。本研究中使用的是低温保存的冻干菌液,并对冻干保存的菌数进行了测定和优化。结果表明,冻干保存的菌液能达到新鲜菌液同等的检验结果。同时,对冻干菌液保存期进行试验验证,发现细菌存活状态稳定。因此,在猪丹毒、多杀性巴氏杆菌病二联灭活疫苗效力检验中,可将新鲜的攻毒菌液冻干保存,用于攻毒保护试验,以避免每次繁重的新鲜菌液前期准备工作,并克服活菌数衰减所造成的检验结果不准确等问题。

In the detection of vaccine efficacy of combined inactivated vaccine against swine erysipelas and Pasteurella multocida,challenge protection test is indispensable in animal models. In traditional method,fresh cultured bacteria,which is temporarily stored at 2 ~ 8 ℃,is always used to challenge animals. In this study,however,the bacteria lyophilized were used as challenge agent and the dose of lyophilized bacteria was measured and optimized in the experiments. The result shows that the lyophilized preserved bacteria liquid has the same effect with the fresh bacterial liquid. Meanwhile,the storage period of freeze-dried bacterial liquid was also verified and the result indicates that viability of bacteria is stable. Therefore,in the efficacy test of vaccine,the fresh cultured bacteria can be lyophilized and stored in the refrigerator as challenge agent which could avoid the repeated,time-consuming and arduous preparatory work and overcome the inaccurate results which may be caused by the attenuation of the viable of the bacteria.