脉冲电刺激联合姜黄素对小鼠足背皮肤黑素瘤的影响

Effect of pulsed electric stimulation combined with curcumin on melanoma of foot back skin in mice

目的:观察脉冲电刺激联合姜黄素对小鼠足背皮肤黑素瘤的影响。方法:将B16-F10小鼠黑素瘤细胞注射到C57/BL6小鼠足背皮下,制备足背黑素瘤模型;脉冲电刺激、姜黄素独立或联合处理黑素瘤,并观察其对肿瘤大小生长情况及对肿瘤细胞凋亡的影响。结果:脉冲电刺激独立或联合姜黄素具有抑制黑素瘤瘤体增大的作用(P<0.01),联合疗法更加有效(P<0.05),且联合治疗组肿瘤细胞凋亡的数量增加(P<0.05)。结论:足背黑素瘤模型便于观察肿瘤形态和测量体积,具有一定优势;脉冲电刺激联合姜黄素可以通过引起细胞凋亡而抑制黑素瘤的生长,是一种有效的治疗黑素瘤的手段。

Objective:To observe the effects of pulsed electric stimulation combined with curcumin on melanoma of foot back skin in mice.Methods:The B16-F10 mouse melanoma cells were subcutaneously injected into the C57/BL6 mouse dorsal foot to establish the dorsal melanoma model.The melanoma was treated with pulsed electric stimulation combined with curcumin,and the tumor size,growth and apoptosis were observed.Results:The pulsed electric stimulation or combined with curcumin could inhibit the tumor growth of melanoma(P<0.01),and the combined therapy was more effective(P<0.05).The number of apoptotic cells increased after combined therapy(P<0.05).Conclusions:The model of dorsal foot melanoma is easy to observe the morphology and measure the volume of the tumor.The pulsed electric stimulation combined with curcumin can inhibit the growth of melanoma by inducing apoptosis,which is an effective treatment method for melanoma.