缝隙连接蛋白26和核转录因子κB在三阴性乳腺癌原发灶及淋巴结转移灶中的表达及临床意义

Expression levels of connexin 26 and NF-κB in primary lesion and metastatic lymph nodes of triple negative breast cancer,and its clinical significance

目的:探讨缝隙连接蛋白26(connexin 26,Cx26)和核转录因子κB(nuclear factorκB,NF-κB)在三阴性乳腺癌(triple negative breast cancer,TNBC)病人原发灶和淋巴结转移灶中的表达及临床意义。方法:回顾性收集经术后病理和分子分型确诊为TNBC的存档石蜡组织,原发灶共49例,其中无区域淋巴结转移者22例(A1组),有区域淋巴结转移者27例(A2组);将27例淋巴结转移灶作为B组。应用免疫组织化学SP染色法,检测各组中Cx26和NF-κB的表达情况,并分析二者表达的相互关系及其与临床病理特征的相关性。结果:原发灶中A2组Cx26和NF-κB阳性表达率均高于A1组(P<0.01和P<0.05);转移灶B组Cx26和NF-κB阳性表达率均高于原发灶A1组(P<0.01)。Cx26蛋白表达与TNBC病人绝经状态、淋巴结转移有关(P<0.01),NF-κB表达与pTNM分期、淋巴结转移有关(P<0.01和P<0.05)。在淋巴结转移灶中,Cx26与NF-κB表达呈明显正相关关系(r=0.663,P<0.01)。结论:Cx26和NF-κB共同参与TNBC的浸润和转移,可作为预测TNBC转移复发及评估临床预后的有效指标。

Objective:To investigate the expression levels of connexin 26(Cx26) and nuckar factor κB(NF-κB) in primary lesion and metastatic lymph nodes of triple negative breast cancer(TNBC),and its clinical significance.Methods:The TNBC paraffin tissues diagnosed by postoperative pathology and molecular classification were collected.Forty-nine cases of primary lesions and 27 cases of lymph nodes metastatic lesions were divided into group A and group B,respectively.Group A included 22 cases without regional lymph node metastasis(group A1) and 27 cases with regional lymph node metastasis(group A2).The expression levels of Cx26 and NF-κB in all cases were examined using immunohistochemical staining.The relationship between Cx26 and NF-κB,and their correlations with clinical features were analyzed.Results:The positive expression rates of Cx26 and NF-κB in group A2 were higher than those in group A1(P<0.01 and P<0.05).The positive expression rates of Cx26 and NF-κB in group B were higher those in group A1(P<0.01).The expression level of Cx26 was correlated with the menopause state and lymph node metastasis(P<0.01),and the NF-κB level was correlated with pTNM staging and lymph node metastasis(P<0.01 and P<0.05).In lymph nodes metastatic lesions,the expression of Cx26 was positively correlated with the expression of NF-κB(P<0.01).Conclusions:Cx26 and NF-κB may co-participate in the invasion and metastasis of TNBC,and can be used as an effective indicator to predict TNBC metastasis and recurrence and evaluate clinical prognosis.