水稻叶片无机磷测定影响因素探究

Analysis of influencing factors for determination of inorganic phosphate concentration in rice leaves

为分析取样部位、取样时间、保存温度、保存时间与苗龄对水稻植株无机磷测定的影响,为水稻无机磷测定提供指导。以水培条件下野生型水稻日本晴(Oryza sativa L.ssp.Japonica cv.Nipponbare)为材料,分别取不同叶序叶片和同一叶片不同部位进行无机磷测定,一天内不同时间取同一叶序叶片进行无机磷测定,相同叶序叶片取样后分别在室温(25℃)和低温(4℃)条件下放置不同时间后进行无机磷测定,取不同苗龄水稻倒二叶进行无机磷测定。对以上测定的磷含量进行比较分析,结果显示,不同取样部位对无机磷测定结果影响较大。叶片从新叶到老叶无机磷浓度逐渐增加,灌浆后的旗叶无机磷浓度较低;同一叶片叶基部无机磷含量最低,叶中部和叶尖无机磷浓度相同;一天内不同时间取样,各时间点之间无机磷浓度没有显著差异;保存温度和保存时间等对水稻叶片无机磷测定结果没有影响。综上,水稻植株无机磷测定时,要保证取样部位一致,其他因素如取样时间、温度、保存时间对结果没有显著影响。

To provide guidance for the measurement of inorganic phosphate concentration,the effect of sampling tissue,sampling time,preservation temperature and seedling age on inorganic phosphate concentration determination of leaves in rice(Oryza sativa L.ssp.Japonica cv.Nipponbare)were analyzed.21-day-old rice seedlings grown under normal rice culture solution were used for inorganic phosphate concentration determination.Different leaves,different parts of a same leaf or leaves of plant in different ages were sampled for inorganic phosphate concentration determination.Effects of preservation temperature and time on phosphate concentration were analyzed.And also,phosphate concentration of leaves sampled at different time in a day was measured.The results indicated that different sampling tissues had great influence on the determination of inorganic phosphorus.Phosphate concentration of old leaves was higher than that of new leaves in the plant,and flag leaves before flowering had a higher inorganic phosphate concentration than these after flowering.The basal part of leaves had a lower phosphate concentration than those of the apex and middle parts of leaves.There was no difference between leaves sampled at different time in a day.Preservation temperature and time had no significant influence in inorganic phosphate concentration determination.These results suggested that sampling tissues or leaves should be consistent when we measure inorganic phosphate concentration.Other factors such as sampling time,temperature and storage time had little effect on the results of inorganic phosphate concentration.