猪丹毒丝菌灭活疫苗免疫佐剂的筛选

Screening of immunological adjuvant for inactivated vaccine against Erysipelothrix rhusiopathiae

为筛选猪丹毒丝菌灭活疫苗免疫佐剂,将5种佐剂(聚合物佐剂、矿物油佐剂ISA 201 VG、蜂胶佐剂、弗氏佐剂、铝胶佐剂)分别与3株猪丹毒丝菌株(AEr21、AEr31和AEr32)制备成15种灭活疫苗,经物理性状检验、无菌检验、安全检验合格后分别免疫小鼠,应用ELISA方法检测小鼠血清中IgG抗体水平和细胞因子含量(IL-4、IL-10、TNF-β、IFN-γ、MCP-1)。通过腹腔注射和灌胃2种方式对免疫后小鼠进行攻毒,计算免疫保护率。结果显示,5种佐剂与3株猪丹毒丝菌株制备的灭活疫苗均可刺激小鼠产生良好的体液免疫和细胞免疫,矿物油佐剂ISA 201 VG疫苗免疫组诱导小鼠产生的IgG抗体水平和细胞因子含量最高,与铝胶佐剂、弗氏佐剂疫苗免疫组差异显著(P<0.05),与聚合物佐剂、蜂胶佐剂疫苗免疫组差异不显著(P>0.05);矿物油佐剂ISA 201 VG疫苗免疫组在攻毒后的免疫保护率最高,对腹腔注射和灌胃攻毒小鼠的免疫保护率分别为80%、80%、60%和100%、100%、80%。试验结果表明,矿物油佐剂ISA 201 VG可作为猪丹毒丝菌灭活疫苗制备的候选免疫佐剂。

To screen out the best immunoadjuvant for Erysipelothrix rhusiopathiae inactivated vaccine,five adjuvants(polymer adjuvant,mineral oil adjuvant ISA 201 VG,propolis adjuvant,Freund s adjuvant,aluminum gel adjuvant)were used to prepare 15 inactivated vaccines with three strains of Erysipelothrix rhusiopathiae(AEr21,AEr31 and AER32),respectively.Mice were immunized by the vaccines which had passed physical property,sterility and safety test,serum antibody IgG and cytokines(IL-4,IL-10,TNF-β,IFN-γ,MCP-1)levels were measured by ELISA.Calculating the protective rate of each immunization group after challenge by intraperitoneal injection and gavage.The results showed that all the inactivated vaccines prepared by five adjuvants and three strains could stimulate mice to produce good humoral and cellular immunity.The levels of IgG and cytokines induced by mineral oil adjuvant ISA 201 VG vaccine in mice were the highest,which was significantly different from that of aluminum gel adjuvant and Freund s adjuvant vaccine(P<0.05),but not significantly different from that of polymer adjuvant and propolis adjuvant vaccine(P>0.05).The mineral oil adjuvant ISA 201 VG vaccine had the highest immune protection rate after challenge,reaching 100%、100%、80%for intraperitoneal injection and 80%、80%、60%for gavage,respectively.The results showed that mineral oil adjuvant ISA 201 VG could be used as a candidate immunoadjuvant for the preparation of inactivated vaccine against Erysipelothrix rhusiopathiae.