一测多评法测定槐花中3种黄酮类成分的含量

Determination of three flavonoids ingredients of Sophorae Flos by QAMS method

目的建立一测多评法(QAMS)用于测定槐花中芦丁,山柰酚 3 O 芸香糖苷及槲皮素3种黄酮类成分的含量。方法以芦丁作为内标物,计算芦丁与槲皮素和山柰酚 3 O 芸香糖苷的相对校正因子,分别采用QAMS和外标法测定40批槐花药材中芦丁,山柰酚 3 O 芸香糖苷和槲皮素的含量,比较两种测定方法之间的差异。结果QAMS与外标法所测得的槐花中芦丁、山柰酚 3 O 芸香糖苷和槲皮素的含量分别为(9.98±1.39)%、(10.08±1.38)%,差异无统计学意义(t=0.324,P=0.747)。结论QAMS准确可行,可用于槐花中3种黄酮类成分测定。

Objective To establish a quantitative analysis of multi components by single marker(QAMS)for the determination of three flavonoids ingredients in Sophorae Flos,i.e.rutin,kaempferol 3 O rutinoside,quercetin.Methods Rutin was used as the ref erence substance,the relative correction factors of kaempferol 3 O rutinoside and quercetin were established,the contents of rutin,kaempferol 3 O rutinoside and quercetin in 40 batches of Sophorae Flos were determined by QAMS and external standard method respectively,and the differences between the two methods were compared.Results The contents of rutin,aempferol 3 O rutinoside and quercetin in Sophorae Flos measured by QAMS and external standard method were(9.98±1.39)%and(10.08±1.38)%,re spectively,with no statistical significance(t=0.324,P=0.747).Conclusion It is feasible and accurate to determine the contents of three flavonoids components in Sophorae Flos by QAMS method.