血管内皮生长因子和骨形态发生蛋白-2在糖尿病病人骨组织内表达水平的研究

Expression of vascular endothelial growth factor and bone morphogenetic protein-2 in bone tissue of diabetic patients

目的探讨血管内皮生长因子(vascular endothelial growth factor, VEGF)、骨形态发生蛋白-2(bone morphogenetic protein-2, BMP-2)在糖尿病病人胫骨组织内的表达水平。方法在胫骨高位截骨术中,分别获取糖尿病病人(8例)和非糖尿病病人(8例)术部骨组织,从骨组织中提取总蛋白及RNA,用Western blot、RT-PCR检测骨组织中VEGF、BMP-2的表达水平。结果糖尿病病人骨组织中VEGF、BMP-2蛋白和mRNA表达水平均明显低于非糖尿病病人。结论糖尿病病人骨组织中VEGF、BMP-2因子表达低于正常水平,VEGF、BMP-2因子可能是影响糖尿病病人骨质及骨愈合的重要因素之一。

Objective To investigate the expression levels of vascular endothelial growth factor(VEGF) and bone morphogenetic protein-2(BMP-2) in the tibia of diabetic patients. Methods In the high osteotomy of the humerus, the bone tissues of diabetic patients(8 cases) and non-diabetic patients(8 cases)were obtained respectively. Total protein and RNA were extracted from bone tissue, and VEGF and BMP-2 in total bone tissue were detected by Western blot/RT-PCR. Results The expression levels of VEGF, BMP-2 mRNA and protein in bone tissue of diabetic patients were significantly lower than those in non-diabetic patients. Conclusion The expression of VEGF and BMP-2 in bone tissue of diabetic patients is lower than normal level. VEGF and BMP-2 may be one of the important factors affecting bone and bone healing in diabetic patients.