髓过氧化物酶与脂蛋白相关磷脂酶A2在心血管疾病诊断中的应用

Application of myeloperoxidase and lipoprotein associated phospholipase A2 in the diagnosis of cardiovascular diseases

目的比较心血管疾病患者与健康对照者血浆髓过氧化物酶(myeloperoxidase,MPO)和脂蛋白相关磷脂酶A2(lipoprotein-associated phospholipase A2,Lp-PLA2)水平的差异,评估MPO和Lp-PLA2在心血管疾病诊断中的作用.方法选取经冠状动脉造影和放射性核素标记等确诊的72例心血管疾病患者编为患病组,将68例健康体检者编为健康对照组.采用酶联免疫法检测两组受试者血浆中的MPO和Lp-PLA2水平,比较两组之间的差异,并对结果进行统计分析.结果通过比较患病组和健康对照组的MPO和Lp-PLA2水平,结果显示两组间有显著的统计学差异,患病的MPO和Lp-PLA2值明显高于健康对照组.ROC曲线显示MPO对心血管病的诊断准确度居于中等偏上,Lp-PLA2对心血管病的诊断准确度较高.结论心血管疾病患者血浆中的MPO和Lp-PLA2含量显著升高,提示MPO和Lp-PLA2对心血管病的具有诊断价值.

Objective To compare plasma levels of myeloperoxidase(MPO)and lipoproteinassociated phospholipase A2(Lp-PLA2)between patients with cardiovascular diseases and healthy controls,and to evaluate the role of MPO and Lp-PLA2 in the diagnosis of cardiovascular diseases.Methods Seventy-two patients with cardiovascular diseases confirmed by coronary angiography and radionuclide labeling were selected as the disease group,and 68 healthy people as the control group.The levels of plasma MPO and Lp-PLA2 of the two groups were measured by enzyme-linked immunosorbent assay(ELISA).The differences between the two groups were compared,and the results were statistical analyzed.Results By comparing the levels of MPO and Lp-PLA2 between the cardiovascular disease and the healthy control groups,the results showed that there was significant differences between the two gooups.The levels of MPO and Lp-PLA2 in the disease group were significantly higher than those in the healthy control group.ROC curve suggested that the diagnostic accuracy of MPO and Lp-PLA2 for cardiovascular diseases was higher than 50%and higher than 90%,respectively.Conclusion The levels of MPO and Lp-PLA2 in the plasma of patients with cardiovascular diseases significantly increases,suggesting their diagnostic value.