摘要
目的:分析miR-133a和miR-208a对急性冠脉综合征(ACS)患者经皮冠状动脉介入(PCI)术后心肌损伤的诊断价值。方法:将2017年6月至2019年1月在邯郸市第一医院行PCI术治疗的83例ACS患者,并根据PCI术后心肌损伤情况分为心肌损伤组41例和非心肌损伤组42例。采用实时荧光定量PCR(RT-PCR)检测患者血清miR-133a和miR-208a表达水平,采用化学发光免疫分析法检测血清肌钙蛋白I(cTnI)水平,Pearson相关法分析患者血清miR-133a和miR-208a表达水平与cTnI水平的相关性,采用受试者工作特征曲线(ROC)评估miR-133a和miR-208a对患者PCI术后心肌损伤的诊断价值。结果:心肌损伤组血清中cTnI水平和miR-133a、miR-208a相对表达量均明显高于非心肌损伤组(P<0.05);患者血清中miR-133a、miR-208a相对表达量分别与cTnI水平呈正相关关系(r=0.741、0.770,P<0.001);miR-133a、miR-208a诊断ACS患者PCI术后心肌损伤的ROC曲线下面积(AUC)分别为0.809(95%CI0.825~0.961)、0.853(95%CI0.890~0.990),其诊断临界值分别为0.95、0.31,敏感性分别为93.60%、95.12%,特异性分别为73.80%、83.33%。结论:miR-133a、miR-208a对ACS患者PCI术后心肌损伤具有较好的诊断敏感性和特异性,是ACS患者PCI术后心肌损伤的有效生物标志物。
Objective:To analyze the diagnostic values of miR-133a and miR-208a in myocardial injury after percutaneous coronary intervention(PCI)in patients with acute coronary syndrome(ACS).Methods:83 patients with ACS who underwent PCI in Handan First Hospital from June 2017 to January 2019 were divided into 41 patients with myocardial injury group and 42 patients with non-myocardial injury group according to the myocardial injury after PCI.Real-time fluorescence quantitative polymerase chain reaction(RT-PCR)was used to detect the levels of serum miR-133a and miR-208a,serum troponin I(cTnI)levels were measured by chemiluminescence immunoassay,Pearson correlation method was used to analyze the correlations between levels of serum miR-133a and miR-208a with cTnI,subject operating characteristic curve(ROC)was used to evaluate the diagnostic value of miR-133a and miR-208a in patients with myocardial injury after PCI.Results:The levels of serum cTnI and the relative expressions of miR-133a and miR-208a in myocardial injury group were significantly higher than those in non-myocardial injury group(P<0.05);the relative expressions of miR-133a and miR-208a in serum of patients were positively correlated with the level of cTnI(r=0.741,0.770,P<0.001);the areas under ROC curve(AUC)of miR-133a and miR-208a in diagnosing myocardial injury after PCI in ACS patients were 0.809(95%CI was 0.825-0.961)and 0.853(95%CI was 0.890-0.990),respectively,the diagnostic thresholds were 0.95 and 0.31,the sensitivity was 93.60%and 95.12%,the specificity was 73.80%and 83.33%,respectively.Conclusion:MiR-133a and miR-208a have good diagnostic sensitivities and specificities for myocardial injury after PCI in ACS patients.They are effective biomarkers of myocardial injury in ACS patients after PCI.
作者
张仁杰
王宁
齐杰
李少明
赵丁峰
常超
ZHANG Renjie(The First Hospital of Handan,Hebei Handan 056000,China)
出处
《河北医学》
CAS
2019年第11期1769-1773,共5页
Hebei Medicine
基金
河北省邯郸市科学技术研究与发展计划项目,(编号:1623208064-4)