早产儿脑白质损伤中辅助性T细胞表达失衡及NF-κB的变化

Expression Imbalance of Helper T cells and the Change of NF-κB in Premature Infants with White Matter Injury

目的:早产儿脑白质损伤(WMI)患儿中辅助性T1细胞及辅助性T2细胞(Th1/Th2)、核因子-κB(NF-κB)的表达情况。方法:选取70例通过头颅MRI诊断为早产儿WMI的患儿为实验组,同期70例无脑白质损伤的早产儿为对照组。进一步将实验组患儿分为轻度、中度和重度WMI组。采用逆转录定量聚合酶链反应(PCR)进行检测Th1/Th2细胞因子的mRNA表达水平,包括白介素-2(IL-2)、肿瘤坏死因子-α(TNF-α)、白介素-4(IL-4)、白介素-10(IL-10)和NF-κB,同时采用ELISA法测定血清蛋白水平。结果:早产儿WMI组,血清IL-4、IL-10水平显著降低,而TNF-α、IL-2和NF-κB水平升高,与对照组有统计学差异(P<0.05)。在重度早产儿WMI组,IL-4、IL-10表达进一步减少,IL-2、TNF-α、NF-κB水平进一步增加(P<0.05)。Th1分泌的IL-2、TNF-α以及NF-κB与Th2分泌的IL-4、IL-10呈负相关。IL-2、TNF-α、NF-κB与疾病严重程度呈正相关,IL-4、IL-10与疾病严重程度呈负相关。结论:Th1/Th2失平衡及NF-κB上调可能是一个潜在的因素参与早产儿WMI发病机制,以便早期诊断和早期治疗。

Objective:To demonstrate the expression of T helper cell 1(Th1),T helper cell 2(Th2)and nuclear factor-κB(NF-κB)in premature infants with white matter injury(WMI).Methods:Serum samples were collected from 70 WMI neonates,plus another control group of 70 premature babies without WMI.Patients were further divided into mild,moderate and severe WMI groups.Reverse transcription quantitative polymerase chain reaction was used to test mRNA expression levels of Th1/Th2 cytokines,including interleukin 2(IL-2),tumor necrosis factor-α(TNF-α),IL-4,IL-10 and nuclear factor(NF)-κB,whilst their serum levels were measured by ELISA.Results:Serum levels of IL-4 and IL-10 were significantly decreased in premature WMI babies,whilst IL-2,TNF-αand NF-κB were upregulated(P<0.05 vs.control group).With aggravated disease,IL-4 and IL-10 expression was further decreased while IL-2,TNF-αand NF-κB were increased(P<0.05 vs.mild WMI group).Th1 cytokines IL-2 and TNF-αand NF-κB were negatively correlated with Th2 cytokines IL-4 and IL-10.Disease severity was positively correlated with IL-2,TNF-αand NF-κB expression,and was negatively correlated with IL-4 and IL-10(P<0.05).Conclusion:Upregulation of Th1/Th2 imbalance NF-kappa B may be a potential factor involved in the pathogenesis of WMI in premature infants for early diagnosis and treatment.