一株高产淀粉酶扣囊复膜孢酵母的产酶条件优化及酶学性质研究

Optimization of enzyme production conditions and enzymatic properties of a high amylase-production Saccharomycopsis fibuligera

对筛选自芝麻香型白酒高温大曲中的1株高产淀粉酶扣囊复膜孢酵母的产酶条件和酶学性质进行研究。在单因素实验的基础上,运用响应面分析,优化得到菌株最佳产酶条件:原料添加量20 g,含水量51%,接种量18%,发酵温度24℃,在此条件下,经72 h固态发酵,淀粉酶活力达到12297 U/g,比优化前提高了约63.96%;进一步对菌株所产淀粉酶性质进行初步研究,该淀粉酶最适作用温度为50℃,最适作用pH值为4.0。实验结果表明,所筛选菌株产淀粉酶能力较强,性能优良,且所产淀粉酶能够适应大曲发酵的酸性高温环境,具有应用于高温大曲生产的潜力。

This paper firstly studied the enzyme production conditions of a high amylase-producing Saccharomycopsis fibuligera screened from high-temperature Daqu of Sesame-flavor Baijiu.Using single factor test and response surface analysis,the optimal conditions were obtained:20 g of raw material,51%water content,18%inoculum and fermented at 24℃.Under the optimal fermentation conditions,the amylase activity reached 12297 U/g after 72 h of solid-state fermentation,which was 63.96%higher than that before optimization.Besides,the enzymatic properties of amylase from this strain were characterized.The optimal reaction temperature was 50℃and the optimal pH was 4.0.The experimental results showed that the strain was effective in producing amylase and the amylase was adaptable to the acidic and high-temperature environment for Daqu fermentation.This strain has the potential in high-temperature Daqu production.