Micro146b在甲状腺乳头状癌中的表达及其对甲状腺乳头状癌侵袭迁移的影响

Expression of miR-146b in thyroid cancer and its role in invasion and migration of thyroid cancer cells

目的:探讨miR-146b在甲状腺乳头状癌中的表达及其对甲状腺乳头状癌侵袭转移的影响。方法:体外培养人甲状腺乳头状癌TPC-1细胞并转染miR-146b,分为4组:miR-146b抑制组及抑制对照组、miR-146b转染组及转染对照组。分别通过qRT-PCR检测miR-146b的表达,CCK-8法检测细胞增殖,Transwell法检测细胞侵袭能力,划痕法检测细胞的迁移能力。结果:MiR-146b在甲状腺乳头状癌TPC-1细胞中高表达(P<0.01)。CCK-8法检测发现miR-146b抑制组TPC-1细胞活力与抑制对照组相比显著降低(P<0.01),而miR-146b转染组细胞活力与转染对照组相比显著提高(P<0.01)。Transwell法检测发现抑制miR-146b后细胞的穿膜数明显减少(P<0.01),而过表达miR-146b后细胞的穿膜数明显增加(P<0.01)。划痕法检测发现miR-146b抑制组的迁移距离与抑制对照组比显著扩大(P<0.01),而miR-146b转染组的迁移距离与对照组比显著缩小(P<0.01)。结论:甲状腺乳头状癌TPC-1细胞中存在miR-146b的高表达。miR-146b的高表达可促进TPC-1细胞的增殖、侵袭及迁移。

Objective:To investigate the expression level of micro RNA-146b in thyroid cancer cell lines and its impact on thyroid cancer invasion and metastasis.Methods:MiR-146b was transfected into human thyroid cancer cells TPC-1 in vitro.All cells were divided into an inhibited miR-146b group,an inhibitor negative control group,a transfected miR-146b group,and a negative control group.MiR-146b expression was detected by qRT-PCR in thyroid cancer cell lines(TPC-1)and negative control cell lines.The characteristics of invasion and migration of the transfected MiR-146b cells were examined by transwell assay.The expression of miR-146b was detected by qRT-PCR,cell proliferation was determined by CCK-8 assay.Migration rate was measured by wound healing assay.Results:MiR-146b was highly expression in thyroid cancer cells TPC-1(P<0.01).CCK-8 assay found that the cell viability in the miR-146b inhibitor group were significantly lower than that in the inhibitor NC group(P<0.01),while that in the miR-146b mimics group were significantly higher than that in the NC group(P<0.01).Transwell assay found that the number of cells through membranes was decreased evidently in the miR-146b inhibitor group(P<0.01),while that was increased evidently in the miR-146b mimics group(P<0.01).The wound healing assay found that the migration distance of miR-146b inhibitor group was farther than that in the inhibitor NC group(P<0.01),while that of the miR-146 mimics group was shorter than that in the NC group(P<0.01).Conclusion:MiR-146b has high expression in thyroid cancer cells TPC-1.The overexpression miR-146b can promote cell proliferation,invasion,and migration.