赭曲霉毒素A检测方法的研究进展

Research progress on detection methods of ochratoxin A

赭曲霉毒素A(ochratoxin A,OTA)是一种具有极端毒性、污染广泛及危害严重的次级代谢产物.由于赭曲霉毒素A具有这些特点且食品安全问题越来越被人们广泛关注,所以确定食品和商品中的OTA污染水平非常重要.目前对于OTA的检测,已经建立了非常多的分析手段,如薄层色谱法(thin layer chromatography,TLC)、高效液相色谱法(high performance liquid chromatography,HPLC)、酶联免疫技术(enzyme linked immunosorlent assay,ELISA)、时间分辨荧光免疫分析技术(time-resolved fluoroimmunoassay,TRFIA)等.本文综述了多种赭曲霉毒素A的检测分析方法,简介了它们的优缺点及研究进展,并进行了比较,以期为食品中赭曲霉毒素A的检测研究提供一些参考.

Ochratoxin A(OTA) is a secondary metabolite with extreme toxicity, extensive contamination and serious hazards. Since ochratoxin A has these characteristics and food safety issues are gaining widespread attention, it is important to determine the level of OTA contamination in foods and commodities. At present, a lot of analytical methods have been established for the detection of OTA, such as thin layer chromatography(TLC), high performance liquid chromatography(HPLC), and enzyme linked immunosorlent assay(ELISA), time-resolved fluoroimmunoassay(TRFIA), and the like. This paper reviews the various detection and analysis methods of ochratoxin A, introduces their advantages and disadvantages and research progress, and compares them to provide some references for the detection of ochratoxin A in food.