摘要
目的 建立基于Au纳米团簇荧光猝灭特性检测酪氨酸酶(tyrosinase,TYR)活性的方法 .方法以谷胱甘肽为模板合成Au纳米团簇(Au nanoclusters AuNCs),通过荧光猝灭检测TYR活性.结果 酪氨酸酶活力范围在11.55~69.30 U/mL时,荧光猝灭减半时间(quenching time of half fluorescence intensity,QTHFI)与酪氨酸酶活力线性相关,r2为0.98,检出限为1.28 U/mL.结论 利用Au纳米团簇荧光特性检测酪氨酸酶活力的新方法可行.
Objective To establish a method of detection of tyrosinase(TYR) activity was researched. Methods AuNCs was synthesized using glutathione(GSH) as the template, and TYR activity was detected by fluorescence quenching. Results There is a good linear relation between the quenching time of half fluorescence intensity(QTHFI) and tyrosinase activity ranging in 11.55-69.30 U/mL, r2 reached 0.98, the detection limit is 1.28 U/mL. Conclusion The method for detecting tyrosinase activity by the fluorescence characteristics of Au nanoclusters is feasible.
作者
闫协民
何蕾
周春霞
洪鹏志
孙省利
李承勇
YAN Xie-Min;HE Lei;ZHOU Chun-Xia;HONG Peng-Zhi;SUN Sheng-Li;LI Cheng-Yong(College of Food Science and Technology,Guangdong Ocean University,Zhanjiang 524088,China;School of Chemistry and Environment,Guangdong Ocean University,Zhanjiang 524088,China;Shenzhen Institute of Guangdong Ocean University,Shenzhen 51810,China)
出处
《食品安全质量检测学报》
CAS
2019年第21期7278-7282,共5页
Journal of Food Safety and Quality
基金
Supported by the National Natural Science Foundation of China(21874029)
Yangfan Program of Guangdong Province(201635018)
Special Support Program of Guangdong Province(2017TQ04N706)
Science and Technology Planning Project of Guangdong Province(2016A020210114)~~
