PRKCq在大鼠损伤坐骨神经组织中的表达及其作用研究

The Study of Protein Kinase-theta(PRKCq) expressions and relative roles in rat sciatic nerve after injury

目的:通过分子生物学方法,分析大鼠坐骨神经损伤后Wallerian溃变过程中,Protein Kinase-theta(PRKCq)在远端神经组织中的表达,进一步通过培养的施旺细胞(Schwann cell,SC),分析PRKCq对体外培养SC的作用,揭示PRKCq在周围神经Wallerian溃变过程中的功能及机制.方法:制备不同时间点损伤大鼠坐骨神经溃变模型,采用Real-time PCR和siRNA的方法,分析PRKCq在大鼠坐骨神经损伤后,不同时间点远端组织中的表达变化,进一步培养和纯化SC,干扰PRKCq在SC中的表达,分析PRKCq对SC增殖和迁移的影响.结果:大鼠坐骨神经损伤后,在Wallerian溃变过程中,远端组织PRKCq的表达显著降低,PRKCq可在培养的SC中表达,PRKCq siRNA干扰SC后,抑制了SC的增殖和迁移.结论:在大鼠坐骨神经损伤后Wallerian溃变过程中,PRKCq的表达显著降低并影响SC的功能,提示PRKCq在神经损伤修复过程中可能发挥一定的作用.

Objective: To explore the expression changes of protein kinase-theta (PRKCq) during Wallerian degeneration (WD) after rat sciatic nerve injury, analyze the roles of PRKCq in cultured Schwann cell (SC). Study the molecular mechanism of PRKCq in the repair and regeneration after nerve injury. Methods: Rat Wallerian degeneration (WD) models were established with sciatic nerve transaction. Expression changes of PRKCq were analyzed by real-time quantitative PCR. The relative functions of PRKCq were analyzed by siRNA in cultured and puri ed SCs. Results: PRKCq were down-regulated after rat sciatic nerve injury during WD processes. PRKCq were expressed in SCs in vivo. PRKCq could reduce SC proliferation and migration by siRNA transfection. Conclusion: PRKCq may play important roles in the process of WD after nerve injury. The study provides a basis of PRKCq on the molecular mechanisms of WD in nerve degeneration and/or regeneration.