鱼腥草不同溶剂提取物的抗病毒活性研究

Study on Antiviral Activity of Different Solvent Extracts of Houttuynia Cordata

目的研究鱼腥草不同溶剂提取物体外抗手足口病毒(enterovirus type 71,EV71)、单纯疱疹病毒1型(herpes simplex virus type 1,HSV-1)、呼吸道合胞病毒(respiratory syncytial virus,RSV)、柯萨奇病毒B3型(coxsackie virus type B3,CV-B3)、柯萨奇病毒B5型(coxsackie virus type B5,CV-B5)的活性。方法采用细胞体外培养技术,建立不同病毒感染模型,用鱼腥草不同溶剂提取物进行治疗,通过细胞融合病变观察法以及MTT比色法检测得到治疗指数(therapeutic index,TI),以此判定其体外抗病毒活性。结果体外抗病毒试验结果证明,鱼腥草不同溶剂提取物对HSV-1、EV71抗病毒作用较强,对RSV、CV-B3、CV-B5的效果甚微或无明显作用。鱼腥草醇提物中黄酮类成分含量较高,抗病毒活性相对较高;30%,50%,75%乙醇提取物以及水提醇沉处理沉淀物对HSV-1的TI值分别是81.68,67.23,41.91,32.61,均高于阳性对照阿昔洛韦TI值23.43,显示鱼腥草水醇提取物抗HSV-1的活性较强;75%,50%,30%乙醇提取物对EV71的TI值分别是19.58,20.13,18.84,效果较为显著。结论研究证实鱼腥草提取物对HSV-1、EV71的抗病毒活性较高,可一定程度为临床应用鱼腥草治疗这2种病毒感染引发的疾病提供参考依据。

OBJECTIVE To study the antiviral activity of different solvent extracts of Houttuynia cordata. These viruses include enterovirus type 71(EV71), herpes simplex virus type 1(HSV-1), respiratory syncytial virus(RSV), coxsackie virus type B3(CV-B3) and coxsackie virus type B5(CV-B5). METHODS A variety of virus infection models were established by in vitro culture technique, and different solvent extracts of Houttuynia cordata were used for treatment. Therapeutic index(TI) was determined by cytopathic effect observation and MTT staining to determine its antiviral activity. RESULTS The results of in vitro antiviral experiments showed that the different solvent extracts of Houttuynia cordata had better antiviral effect on HSV-1 and EV71, and had little or no effect on RSV, CV-B3 and CV-B5. The content of flavonoids in the alcohol extract of Houttuynia cordata was higher, and the antiviral activity was relatively higher. The TI values of HSV-1 for 30%, 50%, 75% ethanol extract and precipitation obtained by water extraction and alcohol precipitation treatment were 81.68, 67.23, 41.91 and 32.61, respectively, and were higher than the positive control acyclovir TI value 23.43. It showed that Houttuynia cordata extract had stronger anti-HSV-1 virus activity. The TI values of 75%, 50%, 30% ethanol extracts for EV71 were 19.58, 20.13 and 18.84, and the effect was significant. CONCLUSION This study confirms that the antiviral activity of Houttuynia cordata extract on HSV-1 and EV71 is higher by in vitro antiviral experiments, which can provide a reference for clinical treatment of diseases caused by these two viruses.