基于网络药理学分析防风-乌梅药对治疗荨麻疹的作用机制

Analysis of Action Mechanism of Divaricate Saposhnikovia Rootsmoked Plum on the Treatment of Urticaria Based on Network Pharmacology

目的基于网络药理学分析防风-乌梅药对治疗荨麻疹的物质基础及分子机制。方法根据文献报道,结合口服利用度≥30%和类药性分析≥0.18,在TCMSP数据库、BATMAN-TCM数据库筛选防风-乌梅药对活性成分及靶点,GeneCards数据库筛选荨麻疹靶点,通过靶点映射确定药对活性成分作用的荨麻疹靶点;借助Cytoscape 3.5.1软件构建活性成分-靶点网络图,STRING平台构建靶蛋白互作网络,应用DAVID数据库对靶点进行KEGG通路分析,利用Cytoscape软件中BiNGO和MCODE插件对靶基因进行GO生物过程富集和聚类分析。结果从防风-乌梅药对中筛选得到槲皮素、山奈酚、汉黄芩素、欧前胡素、升麻素等16个活性成分,作用于IL-6、TNF、SRC、EGFR、IL-8、PTGS2等62个荨麻疹相关靶点,调控TNF信号通路、PI3K/Akt信号通路、NF-kB信号通路、低氧诱导因子1信号通路、NOD样受体信号通路等8条信号通路,参与调节炎症反应、肽-酪氨酸磷酸化、蛋白质分解代谢等生物过程。结论充分揭示了防风-乌梅药对多成分、多靶点、多通路治疗荨麻疹的科学内涵。

OBJECTIVE Based on network pharmacology, the material basis and molecular mechanism of divaricate saposhnikovia rootsmoked plum for the treatment of urticaria were analyzed. METHODS According to literature reports, the active ingredients and targets of divaricate saposhnikovia rootsmoked plum were screened in TCMSP database and BATMAN-TCM database in combination with oral bioavailability(≥30%) analysis and resistance(≥0.18). Urticaria targets were screened in the GeneCards database, and urticaria target with active ingredients were determined by target mapping. Cytoscape 3.5.1 software was used to construct the active component-target network, and the STRING platform was used to construct the target protein interaction network. Biological information annotation database(DAVID) was used to analyze KEGG signaling pathways of the target. The BiNGO and MCODE plug-ins in Cytoscape software were used to enrich and cluster the target genes in the GO biological process. RESULTS Sixteen active ingredients, such as quercetin, kaempferol, wogonin, imperatorin and cimifugin, were screened from the divaricate saposhnikovia rootsmoked plum, acting on 62 urtical-related targets such as Interleukin-6(IL-6), Tumor necrosis factor(TNF), Proto-oncogene tyrosine-protein kinase Src(SRC), Epidermal growth factor receptor(EGFR), Interleukin-8(IL-8), and Prostaglandin G/H synthase 2(PTGS2), regulating eight signaling pathway such as the tumor necrosis factor(TNF) signal channel, phosphoinositide 3 kinase/serine/threonine protein kinase signaling pathway(PI3 K/Akt), nuclear transcription factors-kB signaling pathways(NF-kappa B), HIF-1 signaling pathways(HIF-1), NOD like receptor signaling pathways(NOD), participating in the regulation of inflammatory reactions, peptide-tyrosine phosphorylation, protein catabolism and other major biological processes. CONCLUSION It fully reveals the scientific connotation of divaricate saposhnikovia rootsmoked plum medicine in treating urticaria with multiple components, multiple targets and multiple pathways.