摘要
BACKGROUND Studies have reported that microRNA-30c(miR-30c)has vital functions in the development and progression of multiple cancers.AIM To investigate the clinical significance and role of miR-30c in pancreatic cancer.METHODS MiR-30c and twinfilin 1(TWF1)expression levels were analyzed in Gene Expression Omnibus datasets and validated in human pancreatic cancer by quantitative real-time polymerase chain reaction(RT-qPCR).The effects of miR-30c on pancreatic cancer cell growth,apoptosis,and cell cycle were evaluated by CCK-8 and flow cytometry assays.Furthermore,the in vivo effects were investigated using a subcutaneous xenograft experiment.Target gene prediction software and luciferase reporter assays were used to identify TWF1 as a direct target of miR-30c.RESULTS The expression of miR-30c was significantly decreased in pancreatic cancer tissues and associated with survival.Gain-and loss-of-function assays showed that miR-30c suppressed pancreatic cancer cell proliferation in vitro and in vivo.RT-qPCR,Western blot,and luciferase reporter assays showed that miR-30c directly targeted TWF1.The expression level of miR-30c was negatively correlated with TWF1 expression in pancreatic cancer tissues.Furthermore,the effects of ectopic miR-30c were rescued by TWF1 overexpression.CONCLUSION Our results identified the role of the miR-30c/TWF1 axis in pancreatic cancer progression and demonstrated that miR-30c might serve as a prognostic biomarker and therapeutic target for pancreatic cancer.
BACKGROUND Studies have reported that micro RNA-30 c(mi R-30 c) has vital functions in the development and progression of multiple cancers.AIM To investigate the clinical significance and role of mi R-30 c in pancreatic cancer.METHODS Mi R-30 c and twinfilin 1(TWF1) expression levels were analyzed in Gene Expression Omnibus datasets and validated in human pancreatic cancer by quantitative real-time polymerase chain reaction(RT-q PCR). The effects of mi R-30 c on pancreatic cancer cell growth, apoptosis, and cell cycle were evaluated by CCK-8 and flow cytometry assays. Furthermore, the in vivo effects were investigated using a subcutaneous xenograft experiment. Target gene prediction software and luciferase reporter assays were used to identify TWF1 as a direct target of mi R-30 c.RESULTS The expression of mi R-30 c was significantly decreased in pancreatic cancer tissues and associated with survival. Gain-and loss-of-function assays showed that mi R-30 c suppressed pancreatic cancer cell proliferation in vitro and in vivo.RT-q PCR, Western blot, and luciferase reporter assays showed that mi R-30 c directly targeted TWF1. The expression level of mi R-30 c was negatively correlated with TWF1 expression in pancreatic cancer tissues. Furthermore, the effects of ectopic mi R-30 c were rescued by TWF1 overexpression.CONCLUSION Our results identified the role of the mi R-30 c/TWF1 axis in pancreatic cancer progression and demonstrated that mi R-30 c might serve as a prognostic biomarker and therapeutic target for pancreatic cancer.
基金
Supported by the National Nature Science Foundation of China,No.61802350
