公告

Effects of lef-6 Deletion in Bombyx mori Nucleopolyhedrovirus on Viral Replication,Gene Transcription and Host Cell Apoptosis

为了提高期刊的编辑出版效率,《蚕业科学》期刊已开通官方网站,并启用在线投稿系统,网址为http://www.cylkxjournal.com/cn/cyqk.asp。在线投稿系统包括作者工作区、外审专家工作区和编辑工作区3个子系统,通过网上投稿、网上查稿、网上审稿,实现作者、编辑、审稿专家的一体化在线交流与稿件协同处理。欢迎各位作者、审稿专家登陆访问!

As a member of the late expression factors(LEFs)family,LEF-6 of Bombyx mori nucleopolyhedrovirus(BmNPV)is able to accelerate late gene transcription.In order to study the function of lef-6 gene in the process of viral infection and influence on host cell,we constructed a lef-6-null Bacmid(lef-6-ko-Bacmid)by deleting lef-6 gene with Red recombinant technology,transfected BmN cell,and then ectopia repaired lef-6 by Bac-to-Bac system to constructe a lef-6 repaired Bacmid(lef-6-re-Bacmid).qRT-PCR results showed that deletion of lef-6 gene resulted in decrease in viral genome replication,as well as decline in transcription levels of genes at different stages(early,late and very late).In addition,transcription level of apoptosis suppressor gene iap2 also decreased.Further Western blot analysis showed that overexpression of lef-6 could elevate the expression level of Bcl-2 protein and reduce the expression level of Bax protein to prevent cell apoptosis.In conclusion,deletion of lef-6 gene not only leads to a drop in viral genome replication and gene transcription,but also affects the process of host cell apoptosis by regulating the expression level of Bcl-2 and Bax protein.These results provided new references for exploring the interactive mechanism between BmNPV and silkworm.