荜茇-大黄不同比例配伍对大黄中5种有效成分溶出率的影响

Effect of different proportions of piper chinaroot and rhubarb on the dissolution rate of five effective components in rhubarb

目的探讨荜茇-大黄的不同比例配伍使用对大黄中芦荟大黄素、大黄酸、大黄素、大黄酚、大黄素甲醚溶出率的影响。方法采用HPLC法分析荜茇-大黄不同配伍比例提取液中大黄5种有效成分的含量。色谱柱为Thermo C18柱(250 mm×4.6 mm,5μm),流动相为甲醇-0.1%磷酸溶液,梯度洗脱,流速1 ml/min,柱温30℃,检测波长254 nm。结果芦荟大黄素、大黄酸、大黄素、大黄酚、大黄素甲醚分别在0.0185~0.7418、0.0179~0.7178、0.0159~0.6355、0.0542~2.1672、0.0162~0.6464μg范围内线性关系良好,加样回收率分别为94.35%、95.50%、100.61%、96.27%、97.39%,RSD分别为1.81%、1.99%、2.84%、2.71%、1.86%。与大黄提取液比较,荜茇-大黄(3∶1、2∶1、1∶1、1∶2、1∶3)5个比例配伍后的芦荟大黄素、大黄酸含量均有不同程度提高,大黄酚、大黄素甲醚含量均有不同程度降低,大黄素在1∶1配伍时含量增加,其他比例均降低。结论综合考虑荜茇-大黄的临床应用及5种成分的溶出率,认为荜茇-大黄的最佳配伍比例为1∶1。

Objective To study the effect of different proportions of piper chinaroot and rheum palmatum on the dissolution rate of five effective components(aloe emodin,emodin acid,emodin,emodin,emodin methyl ether).Methods The high performance liquid chromatography(HPLC)method was used to analyz the contents of five effective components of rheum palmatum in the extracts of different combination of piper chinaroot and rheum palmatum.The tests were carried out by Thermo C18(250 mm×4.6 mm,5μm)by gradient elution with methanol and 0.1%phosphoric acid water solution as mobile phase at a flow rate of 1ml/min,the column temperature was 30℃and the detection wavelength was 254 nm.Results The linear ranges of aloe emodin,emodin acid,emodin,emodin,emodin methyl ether were 0.0185-0.7418,0.0179-0.7178,0.0159-0.6355,0.0542-2.1672,0.0162-0.6464μg,respectively.The average recoveries of aloe emodin,emodin acid,emodin,emodin,emodin methyl ether were 94.35%,95.50%,100.61%,96.27%,97.39%,and the RSDs were 1.81%,1.99%,2.84%,2.71%,and 1.86%,respectively.Compared with rheum palmatumby single extract,the content of aloe emodin and emodin were increased by 5 proportions(3:1,2:1,1:1,1:2,1:3),while the content of emodin and emodin methyl ether were decreased.Conclusions The optimal compatibility proportion of piper chinaroot and rheum palmatum is 1:1.