摘要
BACKGROUND Developing mitochondrial regulators/nutrients from natural products to remedy mitochondrial dysfunction represent attractive strategies for therapy of nonalcoholic fatty liver disease(NAFLD).Polygonatum kingianum(PK)has been traditionally used in China as a medicinal and nutritional ingredient for centuries and can alleviate high-fat diet(HFD)-induced NAFLD by promoting mitochondrial functions.To date,the underlying molecular mechanism of PK for treating mitochondrial dysfunctions and thus alleviating NAFLD remains unclear.AIM To identify the molecular mechanism behind the mitochondrial regulatory action of PK against HFD-induced NAFLD in rats.METHODS NAFLD model was induced in rats with HFD.The rats were intragastrically administered PK(4 g/kg per day)for 14 wk.Metabolites in hepatic mitochondrial samples were profiled through ultra-high performance liquid chromatography/mass spectrometry followed by multivariate statistical analysis to find the potential biomarkers and metabolic pathways.RESULTS PK significantly restored the metabolites’levels in the mitochondrial samples.Ten potential biomarkers were identified in the analyzed samples.These biomarkers are involved in riboflavin metabolism.CONCLUSION PK can alleviate HFD-induced NAFLD by regulating the riboflavin metabolism and further improving the mitochondrial functions.Thus,PK is a promising mitochondrial regulator/nutrient for alleviating NAFLD-associated diseases.
BACKGROUND Developing mitochondrial regulators/nutrients from natural products to remedy mitochondrial dysfunction represent attractive strategies for therapy of nonalcoholic fatty liver disease(NAFLD). Polygonatum kingianum(PK) has been traditionally used in China as a medicinal and nutritional ingredient for centuries and can alleviate high-fat diet(HFD)-induced NAFLD by promoting mitochondrial functions. To date, the underlying molecular mechanism of PK for treating mitochondrial dysfunctions and thus alleviating NAFLD remains unclear.AIM To identify the molecular mechanism behind the mitochondrial regulatory action of PK against HFD-induced NAFLD in rats.METHODS NAFLD model was induced in rats with HFD. The rats were intragastrically administered PK(4 g/kg per day) for 14 wk. Metabolites in hepatic mitochondrial samples were profiled through ultra-high performance liquid chromatography/mass spectrometry followed by multivariate statistical analysis to find the potential biomarkers and metabolic pathways.RESULTS PK significantly restored the metabolites’ levels in the mitochondrial samples.Ten potential biomarkers were identified in the analyzed samples. These biomarkers are involved in riboflavin metabolism.CONCLUSION PK can alleviate HFD-induced NAFLD by regulating the riboflavin metabolism and further improving the mitochondrial functions. Thus, PK is a promising mitochondrial regulator/nutrient for alleviating NAFLD-associated diseases.
基金
the National Natural Science Foundation of China,No.81660596
the National Natural Science Foundation of China,No.81760733
the Science and Technology Project of Yunnan China,No.2017FF117-013
the Science and Technology Project of Yunnan China,No.2016FD050
the Application and Basis Research Project of Yunnan China,No.201801CH00227
