胃癌组织中幽门螺杆菌感染和p-p65、EMT相关蛋白的表达及临床意义

H. pylori infection and expression of p-p65 and EMT-related proteins in gastric cancer tissues and their clinical significance

目的探讨胃癌中幽门螺杆菌(H.pylori)感染和p-p65、上皮间质转化(EMT)相关蛋白的表达水平及其临床意义。方法Giemsa染色法检测115例胃癌组织及20例癌旁正常组织标本是否有H.pylori感染,免疫组化SP法测定上述组织中p-p65、Vimentin和E-cadherin的表达,分析H.pylori感染与p-p65、Vimentin和E-cadherin表达的关系,以及与胃癌患者临床病理特征和预后的关系。结果胃癌组织中H.pylori感染率(59.1%)和p-p65、Vimentin阳性表达率(60.0%、34.8%)明显高于癌旁正常组织(30.0%和35.0%、0.0%),E-cadherin阳性表达率(53.9%)明显低癌旁正常组织(100%),差异均具有统计学意义(P<0.05)。胃癌组织中H.pylori感染和p-p65的表达与TNM分期、浸润深度、淋巴结转移及远处转移密切相关(P<0.05);H.pylori感染与p-p65、Vimentin的表达呈显著正相关,与E-cadherin的表达呈显著负相关(P均<0.05)。有H.pylori感染及p-p65和Vimentin阳性表达患者总生存率明显低于阴性表达患者,E-cadherin阴性表达患者总生存率明显低于阳性表达患者(P均<0.05);Cox多因素回归分析显示:TNM分期、淋巴结转移、远处转移、H.pylori感染及E-cadherin表达阴性是影响胃癌患者预后的独立危险因素(P<0.05)。结论在胃癌中H.pylori感染与NF-κB信号通路的活化及EMT的发生密切相关,检测H.pylori感染和p-p65、EMT相关蛋白的表达对判断胃癌患者的临床特征及预后具有一定提示作用。

Objective To investigate the clinical significance of the Helicobacter pylori(H.pylori)infection and the expression levels of p-p65 and epithelial-mesenchymal transition(EMT)-related proteins in gastric cancer.Methods Giemsa staining method was used to detect the presence of H.pylori infection in 115 gastric cancer tissues and 20 paracancerous tissues.Immunohistochemical was performed to determine the expression of p-p65,Vimentin and E-cadherin in the above tissues.The relations of H.pylori infection with p-p65,Vimentin and E-cadherin expressions as well as with clinicopathologic characteristics and prognosis of the patients were analyzed.Results The infection rate of H.pylori(59.1%)and the positive expression rate of p-p65 and Vimentin(60.0%and 34.8%)in gastric cancer tissues were significantly higher than that in paracancerous tissues(30.0%,35.0%and 0.0%),and the positive expression rate of E-cadherin(53.9%)in gastric cancer tissues was significantly lower than that in paracancerous tissues(100%),and the differences were statistically significant(P<0.05).The H.pylori infection and the expression of p-p65 in tumor tissues was closely related to TNM stage,depth of invasion,lymph node metastasis,and distant metastasis(P<0.05).The H.pylori infection in gastric cancer tissues was negatively correlated with the expression of E-cadherin(P<0.05)and positively correlated with the expression of p-p65 and Vimentin(P<0.05).The overall survival rate in patients with H.pylori infection and p-p65,Vimentin positive expression was significantly lower than that in those with negative expression(P<0.05),whereas the overall survival rate in patients with E-cadherin negative expression was significantly lower than that in those with E-cadherin positive expression(P<0.05).Cox regression analysis showed that depth of invasion,lymph node metastasis,distant metastasis,H.pylori infection group,and E-cadherin-negative group expression were the independent prognostic factors of gastric cancer(P<0.05).Conclusion In gastric cancer,H.pylori infection was significantly associated with the activation of NF-κB signaling pathway and the occurrence of EMT.Detecting the H.pylori infection and the expression levels of p-p65 and EMT-related proteins might be helpful in understanding the clinicopathological features and prognosis of patients with gastric cancer.