基于“痰瘀”理论研究心脑宁胶囊对心肌梗死大鼠主动脉钙敏感受体的影响及内皮保护机制

Influences of Xinnaoning capsules on aorta calcium sensing receptor of rats with myocardial infarction and endothelial protection mechanisms based on the theory of phlegm and blood stasis

目的观察心脑宁胶囊对心肌梗死模型大鼠主动脉内膜结构、血清脂联素(APN)、主动脉钙敏感受体(CaSR)表达的影响,探讨心脑宁胶囊保护血管内皮机制。方法 60只雄性SD大鼠,随机选出15只为假手术组,其余45只高脂饲料喂养6周,再进行冠脉结扎手术建立心肌梗死模型,造模成功后将存活大鼠随机分为模型组、西药组、中药组,继续高脂饲料喂养至8周。假手术组大鼠予正常饮食,西药组和中药组分别予阿托伐他汀钙和心脑宁胶囊灌胃,假手术组和模型组均予等量蒸馏水灌胃。用药4周后,HE染色法观察大鼠主动脉内膜结构变化,微板法检测血清甘油三酯(TG)、胆固醇(TC)、低密度脂蛋白(LDL)、高密度脂蛋白(HDL)水平,酶联免疫吸附法(ELISA)检测血清APN水平,免疫蛋白印迹法(Western blot)检测主动脉内CaSR水平变化。结果与假手术组相比,模型组大鼠动脉结构紊乱,内膜有斑块形成,血清TG、TC、LDL和主动脉CaSR表达升高,HDL和APN含量降低(P <0. 05)。与模型组比较,中药组及西药组大鼠动脉内膜较为平滑,血清TG、TC、LDL和主动脉CaSR表达降低,HDL和APN含量升高(P <0. 05);中药组与西药组间各指标差异无统计学意义(P> 0. 05)。结论心脑宁胶囊对心肌梗死模型大鼠具有良好治疗作用。

Objective To observe the impacts of Xinnaoning capsules on the aortic intimal structure,serum levels of diponectin( APN) and calcium sensing receptor( CaSR) expression in rats with myocardial infarction,and explore the endothelial protection mechanisms of Xinnaoning. Methods 15 male Sprague Dawley( SD) rats in sham operation group were randomly selected from 60 rats,the rest 45 rats were fed with high fat diet for 6 weeks and received coronary artery ligation operation. The successful modeling rats were randomly divided into model group,western medicine group and traditional Chinese medicine( TCM) group,and fed with high fat diet for 8 weeks. The sham operation group fed with normal diet. The western medicine group and TCM group received atorvastatin and Xinnaoning capsules,respectively;the sham operation group and model group received same amount of distilled water. After treatment of 4 weeks,the aortic intimal structure was observed by hematoxylin-eosin( HE);the serum levels of triacylglycerol( TG),total cholesterol( TC),low density lipoprotein( LDL) and high density lipoprotein( HDL) were measured by microplate test;serum levels of APN and CaSR expression were determined by enzyme linked immunosorbent assay( ELISA) and Western blot,respectively. Results Compared with sham operation group,aortic intimal structure derangement and plaque formation in intima in model group,and serum levels of TG,TC,LDL and CaSR expression were increased;HDL and APN were decreased( P < 0. 05). Compared with model group,the endarterium was more smooth in the western medicine group and TCM group,serum levels of TG,TC,LDL and CaSR expression were decreased;HDL and APN were increased( P <0. 05). And there were no significant differences between western medicine group and TCM group( P >0. 05). Conclusions Xinnaoning capsules are effective for myocardial infarction.