摘要
目的:建立羊红膻没食子酸,原儿茶酸,新绿原酸,绿原酸,隐绿原酸,木犀草素-7-O-β-D-葡萄糖醛酸苷,异绿原酸A,芹菜素-7-O-β-D-葡糖糖酸苷和异绿原酸C 9种成分的含量测定方法,并研究羊红膻不同药用部位9种成分的含量差异。方法:采用Agilent TC-C18色谱柱(4.6 mm×250 mm,5μm),流动相乙腈-混合酸(0.1%磷酸-0.1%冰乙酸)进行梯度洗脱,切换检测波长分别为265 nm和325 nm,柱温20℃,流速1.0 m L·min^-1。采用Markerlynx XS软件对不同部位含量数据进行分析。结果:定量测定的9种成分没食子酸,原儿茶酸,新绿原酸,绿原酸,隐绿原酸,木犀草素-7-O-β-D-葡萄糖醛酸苷,异绿原酸A,芹菜素-7-O-β-D-葡糖糖酸苷和异绿原酸C分离度良好,在各自线性范围内线性关系良好(r﹥0.9998),精密度、稳定性、重复性试验的RSD均<2.0%,平均回收率99.11%~100.76%,RSD 0.9%~2.0%。结果表明羊红膻不同药用部位化学成分含量差异显著,9种成分的平均含量均表现为在叶部最大,茎部次之,根部相对最小。结论:该研究结果可为羊红膻质量控制、临床应用和资源的合理开发利用提供依据。
Objective:To develop a method to quantify nine constituents in different medicinal parts of Pimpinella thellungiana,in order to compare the content difference of the nine constituents,namely protocatechuic acid,gallic acid,neochlorogenic acid,chlorogenic acid,cryptochlorogenic acid,luteolin-7-O-β-D-glucuronide,isochlorogenic acid A,apigenin-7-O-β-D-glucuronide and isochlorogenic acid C.Method:The analysis was performed on an Agilent TC-C18 column(4.6 mm×250 mm,5μm)with acetonitrile and mixed acid solution(0.1%phosphoric acid-0.1%glacial acetic acid)as mobile phase for gradient elution.The handover detection wavelengths were at 265 and 325 nm.The column temperature was 20℃,and the flow rate was 1.0 m L·min^-1.The experiment data was analyzed using the software of Markerlynx XS.Result:The nine constituents of protocatechuic acid,gallic acid,neochlorogenic acid,chlorogenic acid,cryptochlorogenic acid,luteolin-7-O-β-D-glucuronide,isochlorogenic acid A,apigenin-7-O-β-D-glucuronide and isochlorogenic acid C had a good degree of separation and a good linearity in their respective linear ranges(r>0.9998).The average recoveries ranged from 99.11%to 100.76%,and the RSD ranged from 0.9%to 2.0%.The results showed that the contents of the nine constituents had significant differences in different medicinal parts of P.thellungiana.The average contents of the nine constituents were the highest in leaves,which was followed by stem,and the lowest was in root.Conclusion:The study could provide evidence for the quality control,clinical application,and scientific resources utilization of P.thellungiana.
作者
崔小敏
石会丽
任慧
胡静
蒙麦侠
陈娟
孟雪
陈志永
CUI Xiao-min;SHI Hui-li;REN Hui;HU Jing;MENG Mai-xia;CHEN Juan;MENG Xue;CHEN Zhi-yong(Shaanxi Academy of Traditional Chinese Medicine(TCM),Xi'an 710061,China;Shaanxi TCM Hospital,Xi'an 710003,China)
出处
《中国实验方剂学杂志》
CAS
CSCD
北大核心
2019年第24期97-103,共7页
Chinese Journal of Experimental Traditional Medical Formulae
基金
国家自然科学基金青年基金项目(81603264)
陕西省创新人才推进计划-青年科技新星项目(2018KJXX-073)
陕西省重点研发计划一般项目(2018SF-302)
西安市科技计划项目[201805103YX11SF37(17)]
关键词
羊红膻
不同药用部位
没食子酸
原儿茶酸
新绿原酸
Pimpinella thellungiana
different medicinal parts
protocatechuic acid
gallic acid
neochlorogenic acid