基于Overview和物理图谱的大豆株高性状候选基因挖掘

Candidate Gene Mining of Soybean Plant Height Traits Based on Overview and Physical Map of Soybean Genome

为更加准确地挖掘大豆株高性状候选基因,本研究利用已有研究中与大豆株高性状相关的249个QTL位点,以大豆基因组物理图谱为背景进行整合,并通过Overview分析得到32个重演性较好的置信区间,分布在大豆D1b、N、C1、A1、C2、M、K、O、B1、F、J、D2、G和L连锁群上,其中D1b、A1、C2、M、F、L连锁群的重演性较好的置信区间较多。对候选区段进行基因注释,分析得出植物激素信号转导通路(ID:Ko04075)可能为大豆株高调控的主要通路,该通路与植物细胞增大、分化、茎生长、休眠、果实成熟和抗逆性等植物生理过程紧密相关。通路中13个候选基因与大豆株高性状相关,9个基因被注释为编码生长素响应蛋白,3个基因被注释为编码脱落酸相关蛋白,1个基因为GH3生长素响应启动子。本研究为挖掘大豆株型性状候选基因、构建大豆理想株型和促进分子辅助育种提供新思路。

For more accurate excavation in candidate genes of soybean plant height trait,249 QTLs related to plant height traits were used to integrate base on soybean genome physical map,with the method of Overview analysis,32 confidence intervals with good reproducibility were obtained and located on linkage D1 b,N,C1,A1,C2,M,K,O,B1,F,J,D2,G and L.Among 14 linkage groups,D1 b,A1,C2,M,F and L had more confidence intervals.Gene annotation of the candidate segments yields a plant hormone signal transduction pathway,which was closely related to plant physiological processes such as plant cell enlargement,differentiation,stem growth,dormancy,fruit ripening and stress resistance.The pathway contains 13 candidate genes which closely related soybean plant height traits.Among these 13 genes,9 genes were annotated to encode auxin-responsive proteins,3 genes were annotated to encode abscisic acid-related proteins,and 1 gene was a GH3 auxin-responsive promoter.This study not only shed new light on excavating candidate genes for soybean plant type traits but also construct ideal soybean type and promoting molecular-assisted breeding.