HPLC-MS^n法分析当归极性成分及其在大鼠体内的代谢产物

Analysis of polarity components of Angelicae Sinensis Radix and its metabolites in rats by HPLC-MS^n

该实验通过高效液相色谱-二极管阵列-电喷雾-离子阱-飞行时间质谱联用(HPLC-DAD-ESI-IT-TOF-MS^n)技术,对当归乙醇提取物正丁醇和水溶性部位的主要成分及其在大鼠体内的代谢产物进行分析。采用Agilent Zorbax SB-C18(4.6 mm×250 mm,5μm)的色谱柱,以柱温为28℃,流动相为0.1%甲酸水-甲醇(梯度洗脱),流速为1.0 m L·min^-1,检测波长为280 nm的条件进行样品分析。通过对当归正丁醇和水溶性部位中的成分进行分析,共指认25个化合物,其中11个苯酞糖苷类成分为当归中首次报道;从大鼠含药尿液中共指认出19个化合物,包括2个原形成分和17个代谢产物,其中17个代谢产物为新代谢产物。HPLC-DAD-ESI-IT-TOF-MSn能准确、快速地指认药材提取物中主要成分及生物样品中的代谢产物,该实验的研究结果为阐明当归的药效物质及显效形式(原形、代谢产物或两者兼有)提供了科学依据。

This experiment aims to explore the metabolites of n-butanol and water soluble fraction of an ethanol extracts from Angelicae Sinensis Radix in rats.The chemical constituents of n-butanol and water extracts from Angelicae Sinensis Radix were identified by HPLC-DAD-ESI-IT-TOF-MS^n,and the in vivo metabolites of n-butanol and water extracts were analyzed.By analyzing n-butanol and water extracts from Angelicae Sinensis Radix,25 compounds were detected and identified,in which 11 phthalide glycosides were firstly reported.And 19 compounds were detected and identified in rat urine,including 2 prototype constituents and 17 metabolites,and the 17 metabolites were new compounds.The method can identify the main constituents and metabolites of extracts from traditional Chinese medicine accurately and rapidly,and provide evidence for interpreting effective forms and pharmacodynamics substance(prototype,metabolites,or both)of Angelicae Sinensis Radix.