煜鹤堂蝮蛇酒的降糖降脂和降压作用研究

Antidiabetic,antihyperlipidemic,and antihypertensive effects of Yuhetang Agkistrodon halys medicinal liquor

目的:探究煜鹤堂蝮蛇酒对血糖血脂和血压的调节作用。方法:将检疫合格的SD大鼠、自发性高血压(SHR)大鼠随机分为正常对照组、模型对照组、溶媒对照组及煜鹤堂蝮蛇酒高、中、低剂量组(用药剂量分别相当于成人推荐食用量的20、10和5倍)。SD大鼠除正常对照组外,其余各组大鼠给予定量高脂高糖饲料喂养,同时按10ml/kg给予受试物或溶媒,连续4周,末次给样后检测血糖(GLU)以及血清中总胆固醇(TC)、三酰甘油(TG)、高密度脂蛋白(LDL-C)及低密度脂蛋白(HDL-C)水平。SHR大鼠按10ml/kg灌胃给予受试物4周,每周检测各组大鼠体质量和血压。结果:煜鹤堂蝮蛇酒各剂量均可显著降低高糖高脂模型大鼠GLU和TC水平(P <0. 01),高、中剂量可显著降低模型大鼠TG及LDL-C水平(P <0. 01或P <0. 05);煜鹤堂蝮蛇酒高剂量可明显降低SHR大鼠收缩压(P <0. 05),对实验动物体质量无明显影响。结论:煜鹤堂蝮蛇酒具有明显的降糖调脂和降压作用。

Objective: To investigate the regulatory effect of Yuhetang Agkistrodon halys medicinal liquor on blood glucose,blood lipids,and blood pressure. Methods: Quarantined Sprague-Dawley(SD) rats and spontaneous hypertension rats(SHRs) were randomly divided into normal control group,model control group,solvent control group,and high-,middle-,and low-dose Yuhetang Agkistrodon halys medicinal liquor groups(the dose of the medicinal liquor was 20,10,and 5 times that recommended for adults). All SD rats except those in the normal control group were given a fixed amount of high-fat and high-glucose diet,and the test substance or solvent was given at a dose of 10 ml/kg for 4 consecutive weeks;blood glucose(GLU) and serum levels of total cholesterol(TC),triglyceride(TG),low-density lipoprotein cholesterol(LDL-C),and high-density lipoprotein cholesterol(HDL-C) were measured after the last administration. SHRs were given the test substance by gavage at a dose of10 ml/kg,and body weight and blood pressure were measured every week. Results: Low-,middle-,and high-dose Yuhetang Agkistrodon halys medicinal liquor significantly reduced GLU and TC in the rat model of hyperglycemia and hyperlipidemia(P < 0. 01),while the high-and middle-dose medicinal liquor significant reduced TG and LDL-C in model rats(P < 0. 01 or P < 0. 05). High-dose Yuhetang Agkistrodon halys medicinal liquor significantly reduced systolic pressure in SHRs(P < 0. 05) and had no marked influence on the body weight of experimental animals. Conclusion: Yuhetang Agkistrodon halys medicinal liquor has marked antidiabetic,antihyperlipidemic,and antihypertensive effects.