巴戟天提取物对去卵巢大鼠骨质疏松症的防治作用

Role of Morinda officinalis extract in the prevention and treatment of osteoporosis in ovariectomized rats

目的:探讨巴戟天提取物对实验性骨质疏松症大鼠的影响。方法:健康雄性SD大鼠60只,随机分为假手术组、模型对照组、阳性对照组及巴戟天提取物高、中、低剂量组,每组各10只。对除假手术组外的其余组大鼠通过手术摘除大鼠双侧卵巢建立骨质疏松症模型,造模后分别给予阳性药物碳酸钙D3颗粒0. 5mg/kg,巴戟天提取物高、中、低剂量(0. 36g/kg、0. 18g/kg、0. 09g/kg)干预,假手术组与模型对照组给予等体积蒸馏水,每天1次,共90d,剥离右侧股骨测量股骨中心骨密度与远心端骨密度;称量股骨重量,采用原子吸收分光光度法测定骨钙含量。结果:与假手术组比较,模型对照组大鼠骨密度、骨钙含量、血清雌二醇含量均显著降低,血清碱性磷酸酶含量显著升高,差异均有统计学意义(P <0. 01);与模型对照组比较,巴戟天高剂量组大鼠骨密度及高、中剂量组大鼠骨钙含量、血清雌二醇含量均显著升高,碱性磷酸酶含量显著降低,差异均有统计学意义(P <0. 05或P <0. 01)。结论:巴戟天提取物能够有效阻止骨丢失,促进钙沉积,从而改善骨质疏松症,其作用机制可能与调节雌性激素水平有关。

Objective: To investigate the effect of Morinda officinalis extract on rats with osteoporosis. Methods: A total of 60 healthy female Sprague-Dawley rats were randomly divided into sham-operation group,model control group,positive control group,and high-,middle-,and low-dose Morinda officinalis extract groups,with 10 rats in each group. All rats except those in the sham-operation group were given surgical resection of both ovaries to establish a model of osteoporosis,and after modeling,the rats in the positive control group were given calcium carbonate D3 granules 0. 5 mg/kg,those in the high-,middle-,and low-dose Morinda officinalis extract groups were given high-,middle-,and low-dose Morinda officinalis extract(0. 36,0. 18,and 0. 09 g/kg),and those in the sham-operation group and the model control group were given an equal volume of distilled water,once a day for 90 days. The right femur was isolated to measure the bone mineral density of the center and the distal end of the femur;the weight of the femur was measured,and atomic absorption spectrophotometry was used to measure bone calcium content. Results: Compared with the sham-operation group,the model control group had significant reductions in bone mineral density,bone calcium content,and serum estradiol and a significant increase in serum alkaline phosphatase(P < 0. 01). Compared with the model control group,the high-dose Morinda officinalis extract group had significant increases in bone mineral density,bone calcium content,and serum estradiol and a significant reduction in serum alkaline phosphatase,and the middle-dose Morinda officinalis extract group had significant increases in bone calcium content and serum estradiol and a significant reduction in serum alkaline phosphatase(P < 0. 05 or P < 0. 01). Conclusion: Morinda officinalis extract can effectively inhibit bone loss,promote calcium deposition,and thus improve osteoporosis,possibly by regulating the level of estrogen.