罗丹明类染料荧光猝灭法测定异烟肼

Determination of isoniazid by rhodamine dye fluorescence quenching method

在HAc-NaAc缓冲溶液中,罗丹明类染料罗丹明6G(Rh6G)、罗丹明B(RhB)和丁基罗丹明(b-RhB)分别在553,582和589 nm处产生一个较强的荧光峰。加入异烟肼(INH)后,由于异烟肼分别与Rh6G,RhB和b-RhB发生作用,从而使得3种体系的荧光均发生猝灭。在最佳实验条件下,异烟肼浓度分别在0.10~20μg/mL,0.10~25μg/mL和0.50~22μg/mL范围内与各体系的荧光强度改变值(!F)之间呈良好的线性关系,且3种体系的检出限分别为9.04,21.7和20.4 ng/mL。据此,建立了一种荧光猝灭法测定药物中异烟肼含量的分析方法。

In the HAc-NaAc acidic buffer solution,Rhodamine 6 G(Rh6 G),Rhodamine B(RhB)and Butyl Rhodamine B(b-RhB)can produce a strong fluorescence peak at 553,582 and 589 nm,respectively.After the addition of isoniazid(INH),as isoniazid can interact with Rh6 G,RhB and bRhB,respectively,fluorescence quenching of the three systems occurs.Under the optimal experimental conditions,there was a good linear relationship between the fluorescence signal and the concentration of isoniazid in the range of 0.10-20,0.10-25,0.50-22μg/mL,respectively,and the detection limits were 9.04,21.7,20.4 ng/mL,respectively.Therefore,a fluorescence quenching method for the rapid determination of isoniazid in drugs was developed.