环状RNA在口腔鳞状细胞癌中的作用探讨

Investigation of circular RNA in oral squamous cell carcinoma

目的:探讨环状RNA在口腔鳞癌中的作用。方法:选取8对口腔鳞癌组织,采用高通量测序,检测环状RNA在口腔鳞癌中的表达。挑选出差异表达的环状RNA,采用qRT-PCR技术对40对口腔鳞癌及癌旁组织进行验证。采用GraphPad Prism 5.0软件,通过t检验得到表达有显著差异的环状RNA,利用生物信息学方法预测差异表达的环状RNA在口腔鳞癌中的可能作用。结果:口腔鳞癌中存在大量环状RNA。经qRT-PCR验证,口腔鳞癌组织中hsa_circ_0093229、hsa_circ_0006208和hsa_circ_0006677显著下调。经生物信息学分析,此3种环状RNA可能吸附miRNA,在口腔鳞癌中起调控作用。结论:口腔鳞癌中存在大量环状RNA。首次发现hsa_circ_0093229、hsa_circ_0006208和hsa_circ_0006677可能参与口腔鳞癌调控,为口腔鳞癌生物标志物的研究提供了新的方向。

PURPOSE:Circular RNA(circRNA)is a special type of non-coding RNA,which is highly conserved and stable.CircRNAs have been reported to be closely associated with the development of various cancers.However,the role of circRNA in oral squamous cell carcinoma(OSCC)remains unclear.This research aimed to investigate the role of circRNAs in OSCC.METHODS:The circRNA expression profiles were obtained by screening 8 pairs of snap-frozen OSCC and paired adjacent normal tissues via high-throughput sequencing.The expression levels of identified circRNAs were further verified in 40 pairs of OSCC tissues by qRT-PCR.Through GraphPad Prism 5.0 analysis,the significantly expressed circRNAs(P<0.05)were selected for further investigation.CircRNA-microRNA(miRNA)network was predicted through various bioinformatics databases.RESULTS:High-throughput sequencing results showed circRNAs were abundantly expressed in OSCC tissues.Hsa_circ_0093229,hsa_circ_0006208 and hsa_circ_0006677 were significantly down-regulated in the cancer tissues.Bioinformatics analysis showed that hsa_circ_0093229,hsa_circ_0006208 and hsa_circ_0006677 might be novel biomarkers of OSCC.CONCLUSIONS:The results revealed that OSCC tissues have abundant circRNAs.Down-regulated hsa_circ_0093229,hsa_circ_0006208 and hsa_circ_0006677 might be novel biomarkers for OSCC.